LSSR1 facilitates seed setting rate by promoting fertilization in rice

文献类型： 外文期刊

第一作者： Xiang, Xiaojiao

作者： Xiang, Xiaojiao;Zhang, Peipei;Yu, Ping;Zhang, Yingxin;Yang, Zhengfu;Sun, Lianping;Wu, Weixun;Khan, Riaz Muhammad;Abbas, Adil;Cheng, Shihua;Cao, Liyong;Xiang, Xiaojiao;Zhang, Peipei;Yu, Ping;Zhang, Yingxin;Yang, Zhengfu;Sun, Lianping;Wu, Weixun;Khan, Riaz Muhammad;Abbas, Adil;Cheng, Shihua;Cao, Liyong;Xiang, Xiaojiao;Yang, Zhengfu

作者机构：

关键词： Cellulase; Microsporogenesis; Secretory protein; CRISPR-Cas9 system; Spikelet sterility

期刊名称：RICE （ 影响因子：4.783； 五年影响因子：5.23 ）

ISSN： 1939-8425

年卷期： 2019 年 12 卷

页码：

收录情况： SCI

摘要： Seed setting rate is one of the major components that determine rice (Oryza sativa L.) yield. Successful fertilization is necessary for normal seed setting. However, little is known about the molecular mechanisms governing this process. In this study, we report a novel rice gene, LOW SEED SETTING RATE1 (LSSR1), which regulates the seed setting rate by facilitating rice fertilization. LSSR1 encodes a putative GH5 cellulase, which is highly conserved in plants. LSSR1 is predominantly expressed in anthers during the microsporogenesis stage, and its encoded protein contains a signal peptide at the N-terminal, which may be a secretory protein that stores in pollen grains and functions during rice fertilization. To explore the physiological function of LSSR1 in rice, loss-of-function mutants of LSSR1 were created through the CRISPR-Cas9 system, which showed a significant decrease in rice seed setting rate. However, the morphology of the vegetative and reproductive organs appears normal in lssr1 mutant lines. In addition, lssr1 pollen grains could be normally stained by I-2-KI solution. Cytological results demonstrate that the blockage of fertilization mostly accounted for the low seed setting rate in lssr1 mutant lines, which was most likely caused by abnormal pollen grain germination, failed pollen tube penetration, and retarded pollen tube elongation. Together, our results suggest that LSSR1 plays an important role in rice fertilization, which in turn is vital for maintaining rice seed setting rate.

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