Simultaneous detection and differentiation of canine parvovirus and feline parvovirus by high resolution melting analysis

文献类型： 外文期刊

第一作者： Sun, Yaru

作者： Sun, Yaru;Cheng, Yuening;Lin, Peng;Zhang, Hewei;Yi, Li;Tong, Mingwei;Cao, Zhigang;Li, Shuang;Cheng, Shipeng;Wang, Jianke;Sun, Yaru;Cheng, Yuening;Lin, Peng;Zhang, Hewei;Yi, Li;Tong, Mingwei;Cao, Zhigang;Li, Shuang;Cheng, Shipeng;Wang, Jianke

作者机构：

关键词： Simultaneous detection; Differentiation; Canine parvovirus; Feline parvovirus; HRM

期刊名称：BMC VETERINARY RESEARCH （ 影响因子：2.741； 五年影响因子：2.955 ）

ISSN： 1746-6148

年卷期： 2019 年 15 卷

页码：

收录情况： SCI

摘要： BackgroundCanine parvovirus (CPV) and feline parvovirus (FPV) are causative agents of diarrhea in dogs and cats, which manifests as depression, vomiting, fever, loss of appetite, leucopenia, and diarrhea in young animals. CPV and FPV can single or mixed infect cats and cause disease. To diagnose sick animals effectively, an effective virus diagnostic and genome typing method with high sensitivity and specificity is required.ResultsIn this study, a conserved segment containing one SNP A4408C of parvovirus was used for real-time PCR amplification. Subsequently, data were auto-analyzed and plotted using Applied Biosystems (R) High Resolution Melt Software v3.1. Results showed that CPV and FPV can be detected simultaneously in a single PCR reaction. No cross-reactions were observed with canine adenovirus, canine coronavirus, and canine distemper virus. The assay had a detection limit of 4.2 genome copies of CPV and FPV. A total of 80 clinical samples were subjected to this assay, as well as to conventional PCR-sequence assay and virus isolation. Results showed that the percentage of agreement of the assay and other methods are high.ConclusionsIn short, we have developed a diagnostic test for the accurate detection and differentiation of CPV and FPV in fecal samples, which is also cost effective.

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