Comparative Transcriptome Analysis of Celery Leaf Blades Identified an R2R3-MYB Transcription Factor that Regulates Apigenin Metabolism

文献类型： 外文期刊

第一作者： Yan, Jun

作者： Yan, Jun;Yu, Li;He, Lizhoung;Zhu, Longying;Xu, Shuang;Wan, Yanhui;Wang, Hong;Wang, Ying;Zhu, Weimin

作者机构：

关键词： celery; leaf blade; apigenin; comparative transcriptome; R2R3-MYB transcription factor; overexpression and antisense expression; positive regulation

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

ISSN： 0021-8561

年卷期： 2019 年 67 卷 18 期

页码：

收录情况： SCI

摘要： Apigenin has been proven to possess many pharmacological properties, but the mechanism of regulation of apigenin biosynthesis in plants remains unclear. Apigenin is the main flavonoid in celery and is mainly accumulated in the middle stage of leaf blade development. In this study, comparative transcriptomic analysis revealed a large number of structural genes and transcription factor genes that may be involved in the apigenin metabolic pathway. On the basis of the apigenin content in different celery accessions, an R2R3-MYB transcription factor gene, named AgMYB1, was isolated from the high apigenin celery accession C014. Bioinformatics analysis indicated that AgMYB1 may be involved in flavonoid metabolism. AgMYB1 expression showed a positive relation with the expression of the apigenin accumulation marker gene FNSI and with the apigenin content in different celery tissues. Moreover, overexpression and antisense expression of AgMYBI in transgenic celery plants significantly increased and reduced the expression of apigenin biosynthetic genes and the apigenin content, respectively. These findings suggest that AgMYB1 is involved in positive regulation of apigenin metabolism in celery.

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