Expression profiles of sex-related genes in gonads of genetic male Takifugu rubripes after 17 beta-estradiol immersion

文献类型： 外文期刊

第一作者： Hu Peng

作者： Hu Peng;Liu Bin;Ma Qian;Liu Shufang;Liu Xinfu;Zhuang Zhimeng;Hu Peng;Ma Qian;Liu Shufang;Zhuang Zhimeng;Hu Peng;Ma Qian;Liu Shufang;Zhuang Zhimeng

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关键词： Takifugu rubripes; ovary-to-testis recovery; sex-related genes; mRNA expression

期刊名称：JOURNAL OF OCEANOLOGY AND LIMNOLOGY （ 影响因子：1.265； 五年影响因子：1.276 ）

ISSN： 2096-5508

年卷期： 2019 年 37 卷 3 期

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收录情况： SCI

摘要： Estradiol treatment during early life stages of tiger puffer Takilugu rubripes induces feminization in genetic males. However, the ovaries in genetic males may revert to testes once estradiol treatment is halted. Therefore studies should investigate molecular mechanisms underlying ovary-to-testis recovery in genetic males after treatment. In the present study, tiger puffer were exposed to 10, and 100 mu g/L 17 beta-estradiol (E-2) from 15 to 100 days post-hatching (dph), then gonad phenotypes and expression profiles of six sex-related genes (cyp19a, foxl2, dmrt1, amh, sox9a, and sox9b) were characterized after the exposure. Results showed that both 10 and 100 mu g/L E-2, induced ovarian development in genetic males at 100 dph. However, all ovaries induced by 10 mu g/L E-2 first developed into intersexual gonads and subsequently reverted to testes after the exposure. As for treatment of 100 mu g/L E-2, while the rest of the ovaries maintained morphological stability, percentages of intersexual gonads reached 38%-57%, and none were reverted to testes. Increased mRNA levels of cyp19a, foxl2 and sox9b and decreased mRNA levels of dmrt1, amh, and sox9a were observed during the ovarian development in genetic males. While contrary gene expression profiles were detected during ovary-to-testis transformation. The mRNA levels of all the six genes were increased during the development of intersexual gonads. These results indicated that up-regulation of dmrt1, amh and sox9a is associated with initial ovary-to-intersexual transformation, and suppression of-foxl2, cyp19a and sox9b is essential for complete ovary-to-testis recovery in genetic males. This research will help to trace the molecular processes underlying gonadal transformation in teleosts.

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