Metabolite profiling in two contrasting Tibetan hulless barley cultivars revealed the core salt-responsive metabolome and key salt-tolerance biomarkers

文献类型： 外文期刊

第一作者： Wang, Yulin

作者： Wang, Yulin;Zeng, Xingquan;Xu, Qijun;Yuan, Hongjun;Jiabu, Dunzhu;Sang, Zha;Wang, Yulin;Zeng, Xingquan;Xu, Qijun;Yuan, Hongjun;Jiabu, Dunzhu;Sang, Zha;Nyima, Tashi;Mei, Xiao;Nyima, Tashi

作者机构：

关键词： Biomarkers; hulless barley; salinity stress; tolerance mechanisms; widely targeted metabolites

期刊名称：AOB PLANTS （ 影响因子：3.276； 五年影响因子：3.496 ）

ISSN： 2041-2851

年卷期： 2019 年 11 卷 2 期

页码：

收录情况： SCI

摘要： Salinity stress represents one of the most harmful abiotic stresses for agricultural productivity. Tibetan hulless barley is an important economic crop widely grown in highly stressful conditions in the Qinghai-Tibet Plateau and is often challenged by salinity stress. To investigate the temporal metabolic responses to salinity stress in hulless barley, we performed a widely targeted metabolomic analysis of 72 leaf samples from two contrasting cultivars. We identified 642 compounds 57 % of which were affected by salt stress in the two cultivars, principally amino acids and derivatives, organic acids, nucleotides, and derivatives and flavonoids. A total of 13 stress-related metabolites including piperidine, L-tryptophan, L-glutamic acid, L-saccharopine, L-phenylalanine, 6-methylcoumarin, cinnamic acid, inosine 5-monophosphate, aminomalonic acid, 6-aminocaproic acid, putrescine, tyramine and abscisic acid (ABA) represent the core metabolome responsive to salinity stress in hulless barley regardless of the tolerance level. In particular, we found that the ABA signalling pathway is essential to salt stress response in hulless barley. The high tolerance of the cultivar 0119 is due to a metabolic reprogramming at key stress times. During the early salt stress stages (0-24 h), 0119 tended to save energy through reduced glycolysis, nucleotide metabolism and amino acid synthesis, while increased antioxidant compounds such as flavonoids. Under prolonged stress (48-72 h), 0119 significantly enhanced energy production and amino acid synthesis. In addition, some important compatible solutes were strongly accumulated. By comparing the two cultivars, nine salt-tolerance biomarkers, mostly unreported salt-tolerance compounds in plants, were uncovered. Our study indicated that the salt tolerant hulless barley cultivar invokes a tolerance strategy which is conserved in other plant species. Overall, we provide for the first time some extensive metabolic data and some important salt-tolerance biomarkers which may assist in efforts to improve hulless barley tolerance to salinity stress. Tibetan hulless barley is an important economic crop widely grown in highly stressful conditions in the Qinghai-Tibet Plateau and is often challenged by salinity stress. We investigated the temporal metabolic responses to salinity stress by employing the widely targeted metabolomic analysis in leaf samples from two contrasting cultivars. We successfully identified 13 stress-related metabolites representing the core metabolome responsive to salinity stress. Moreover, we revealed that the efficient management of glycolysis and energy consumption, especially during the most stressful time is the key strategy for tolerating salt stress. Finally, some unreported salt-tolerance biomarkers were unveiled in hulless barley.

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