Identification of a novel transcription factor under long-term drought resistance in highland barley: a DNA affinity purification sequencing-based transcriptomic analysis

文献类型： 外文期刊

第一作者： Wang, Yulin

作者： Wang, Yulin;Zhao, Chuansen;Yang, Chunbao;Xu, Qijun;Yuan, Hongjun;Yang, Haizhen;Zeng, Xingquan;Wang, Yulin;Yang, Chunbao;Xu, Qijun;Yuan, Hongjun;Yang, Haizhen;Zeng, Xingquan;Li, Heng

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关键词： DAP-sequencing; GATA family; Highland barley drought resistance; HOVUSG2784400; Transcriptome factors

期刊名称：CHEMICAL AND BIOLOGICAL TECHNOLOGIES IN AGRICULTURE （ 影响因子：6.6； 五年影响因子：6.6 ）

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年卷期： 2023 年 10 卷 1 期

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收录情况： SCI

摘要： Stress emanating from drought condition is one of the inevitable conditions that occurs in many environments and spread across borders and results in severe hindrance to the morphology, physiology, biochemistry and molecular attributes of plants. Highland barley drought tolerance has been demonstrated to be a polygenic related characteristic and genetic composition that can assist in dissecting the gene network(s) controlling the drought tolerance needs to be adequately understood. DNA affinity purification sequencing (DAP-seq) has been shown to contribute to ways of making pure some of the protein with rare sequence-specific DNA binding and can help understand the molecular dynamics in barley under varying exposure time to drought. The present study aimed at identifying novel transcription factors (TFs) in highland barley that are key in drought resistance through DAP-seq-based transcriptomic analysis. The experimental design included two hulless barley accessions; drought-resistant Sheera 10 (X) and drought-sensitive 5171-7 (W), that were both subjected to short-term (4 h) and long-term (48 h) exposure to drought (T1 and T5, respectively), with the control group (CK) involving not subjecting the two accessions to any treatment. Through transcriptome analysis, one candidate transcription factor (GATA family; (bHLH, MYB-related, GARP-G2-like, bZIP, HB-HD-ZIP, C2H2, SET, mTERF, AP2/ERF-ERF, ARID, NAC, GARP-ARR-B, C2C2-GATA, FAR1, Trihelix, NF-YB, B3 and AUX/IAA) was found. The motif obtained was found to be consistent with GATA transcription factor. The DAP-seq highlighted the differential expression target gene which were verified by RT-qPCR. The associated genes were found to be a cluster of structural gene IP_HOR_1, IP_HOR_2 and In_HOR locus. Through RT-qPCR, it was also proved that the gene expressions were indeed upregulated. The TF HOVUSG2784400 was confirmed to be responsible to drought stress under long-term exposure, which regulates the differential expression of the genes, thereby improving the drought resistance of barley.

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