Determination of deoxynivalenol by ELISA and immunochromatographic strip assay based on monoclonal antibodies
文献类型： 外文期刊
第一作者： Yon, Tingting
作者： Yon, Tingting;Zhang, Qi;Wang, Du;Li, Peiwu;Tang, Xiaoqian;Zhang, Wen;Yon, Tingting;Zhang, Qi;Wang, Du;Li, Peiwu;Tang, Xiaoqian;Zhang, Qi;Li, Peiwu;Zhang, Wen;Zhang, Qi;Li, Peiwu;Zhang, Wen
作者机构：
关键词： Deoxynivalenol; monoclonal antibody; indirect competitive enzyme-linked immunosorbent assay (ic-ELISA); immunochromatographic strip (ICS)
期刊名称：TOXIN REVIEWS （ 影响因子：4.266； 五年影响因子：3.581 ）
ISSN： 1556-9543
年卷期：
页码：
收录情况： SCI
摘要： Deoxynivalenol (DON) has numerous toxicological effects. In this study, a monoclonal antibody (mAb) against DON with IC50 7.12 ng/mL was prepared, which was highly specific to trichothecene, except for 3-AC-DON. A quantitative indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and qualitative nanogold probe-based immunochromatographic strip (ICS) assay were established to detect DON. The analytical range of ic-ELISA was 0.90-83.07 ng/mL, and the limit of detection (LOD) were 0.20 ng/mL, 12.5 ng/mL for ic-ELISA and ICS, respectively. The results showed that the ic-ELISA and ICS based on the anti-DON mAb could be efficaciously applied to analyze agricultural products with DON.
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