A Disposable Aptasensor for Sensitive Detection of Okadaic Acid in Shellfish

文献类型： 外文期刊

第一作者： Chen Jia-Qi

作者： Chen Jia-Qi;Wu Hai-Yan;Zhang Xu-Zhi;Zheng Guan-Chao;Sun Xiao-Jie;Guo Meng-Meng;Tan Zhi-Jun;Zhai Yu-Xiu;Chen Jia-Qi;Mu Hai-Jin

作者机构：

关键词： Okadaic acid; Aptasensor; Electrochemical impedance spectroscopy; Screen printed gold electrode; Sensitive detection

期刊名称：CHINESE JOURNAL OF ANALYTICAL CHEMISTRY （ 影响因子：1.134； 五年影响因子：0.909 ）

ISSN： 0253-3820

年卷期： 2019 年 47 卷 6 期

页码：

收录情况： SCI

摘要： Based on the specific combination between aptamer and its target analyte, a disposable aptasensor was built for the sensitive detection of okadaic acid ( OA ) ( a main constituent of diarrhetic shellfish poisoning) in shellfish. The aptamer was employed as the probe and screen-printed gold electrodes ( SPGE ) as the support. Electrochemical impedance spectroscopy ( EIS) was performed to test the change of electron transfer resistances ( R-ct) of SPGE before and after binding with OA and OA in shellfish samples could be thus quantified. In order to improve the sensitivity of the method, Fe3O4@Au was introduced into the fabrication of SPGE since it could enhance the gold working electrode's surface areas. Compared with antibody, the use of aptamer was cheaper, and SPGE avoided the polish step which was needed of traditional electrodes. The change of Ret was linearly correlated with the natural logarithm of concentration of OA from 1 mu g/kg to 800 mu g/kg. The limit of detection was 1 mu g/kg. Recoveries in diarrhetic shellfish at different spiked levels were in the range of 92.0% -118. 6%, and relative standard deviations ( RSDs) were less than 12%. The proposed disposable aptasensor was efficient and sensitive with good repeatability, and was suitable for screening and quantification of shellfish samples.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序