Cytological and proteomic analyses of floral buds reveal an altered atlas of meiosis in autopolyploid Brassica rapa

文献类型： 外文期刊

第一作者： Yang, Yan

作者： Yang, Yan;Wei, Fang;Tian, Baoming;Shi, Gongyao;Cao, Gangqiang;Yang, Yan;Wei, Fang;Braynen, Janeen;Yuan, Jiachen;Braynen, Janeen;Wei, Xiaochun;Zhang, Xiaowei

作者机构：

关键词： Meiosis; Proteome; Floral buds; Autopolyploid; Brassica rapa

期刊名称：CELL AND BIOSCIENCE （ 影响因子：7.133； 五年影响因子：6.302 ）

ISSN： 2045-3701

年卷期： 2019 年 9 卷

页码：

收录情况： SCI

摘要： BackgroundPolyploidy is considered as a basic event in plant speciation and evolution in nature, and the cytological and proteomic profilings of floral buds at meiosis (FAM) would definitely contribute to a better understanding of the polyploid-associated effects during plant reproduction cycle.ResultsHerein, the cytological investigations demonstrated that chromosome behaviors such as univalent and multivalent at prophase I, chaotic alignments at metaphase, aberrant segregation at telophase, were frequently observed during meiosis in autotetraploid Brassica rapa. The proteomic analysis showed a total of 562 differentially expressed proteins (DEPs) were identified in FAM between autotetraploid and diploid B. rapa. Notably, PARP2 and LIG1 related to base excision repair and BARD1 involved in recombination were significantly down-regulated in autotetraploid B. rapa, which indicated DNA repair pathway were more likely affected during meiosis in autotetraploid B. rapa. The functional analysis showed that DEPs assigned to chromatin structure and dynamics, cell cycle control, cell division, chromosome partitioning and cytoskeleton were preferentially up-regulated, which suggested a robust regulation of cell division in autotetraploid B. rapa. In combination with the floral RNA-seq data released, a number of DEPs were found positively correlated with their transcript abundance, but posttranslational modification of proteins might also play a role in regulating meiosis course after polyploidization.ConclusionsIn general, this study provides a detailed cytology and proteome landscape of FAM between diploid and autotetraploid B. rapa, which definitely affords us a better understanding of uniformity and discrepancy of meiosis at the plant reproductive stage before and after polyploidization.

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