Chromosomal deletions mediated by CRISPR/Cas9 in Helicoverpa armigera

文献类型： 外文期刊

第一作者： Jin, Ming-Hui

作者： Jin, Ming-Hui;Xiao, Yu-Tao;Wu, Kong-Ming;Jin, Ming-Hui;Xiao, Yu-Tao;Cheng, Ying;Hu, Jie;Xue, Chao-Bin

作者机构：

关键词： chromosomal deletion; CRISPR; Cas9; genome editing tool; Helicoverpa armigera; knockout; sgRNAs

期刊名称：INSECT SCIENCE （ 影响因子：3.262； 五年影响因子：3.206 ）

ISSN： 1672-9609

年卷期： 2019 年 26 卷 6 期

页码：

收录情况： SCI

摘要： Helicoverpa armigera, cotton bollworm, is one of the most disastrous pests worldwide, threatening various food and economic crops. Functional genomic tools may provide efficient approaches for its management. The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system, dependent on a single guide RNA (sgRNA), has been used to induce indels for targeted mutagenesis in cotton bollworm. However, genomic deletions may be more desirable to disrupt the function of noncoding genes or regulatory sequences. By injecting two sgRNAs with Cas9 protein targeting different exons, we obtained predictable genomic deletions of several hundred bases. We achieved this type of modification with different combinations of sgRNA pairs, including HaCad and HaABCC2. Our finding indicated that CRISPR/Cas9 can be used as an efficient tool to engineer genomes with chromosomal deletion in H. armigera.

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