The ubiquitin-proteasome system is necessary for the replication of duck Tembusu virus

文献类型： 外文期刊

第一作者： Han, Kaikai

作者： Han, Kaikai;Zhao, Dongmin;Liu, Yuzhuo;Liu, Qingtao;Huang, Xinmei;Yang, Jing;Zhang, Lijiao;Li, Yin

作者机构：

关键词： Duck tembusu virus; Ubiquitin-proteasome system; Viral replication

期刊名称：MICROBIAL PATHOGENESIS （ 影响因子：3.738； 五年影响因子：3.663 ）

ISSN： 0882-4010

年卷期： 2019 年 132 卷

页码：

收录情况： SCI

摘要： Duck Tembusu virus (DTMUV) is a newly emerging pathogenic flavivirus that has caused massive economic losses to the duck industry in China. The cellular factors required for DTMUV replication have been poorly studied. The ubiquitin-proteasome system (UPS), the major intracellular proteolytic pathway, mediates diverse cellular processes, including endocytosis and signal transduction, which may be involved in the entry of virus. In the present study, we explored the interplay between DTMUV replication and the UPS in BHK-21 cells and found that treatment with proteasome inhibitor (MG132 and lactacystin) significantly decreased the DTMUV progency at the early infection stage. We further revealed that inhibition of the UPS mainly occurs on the level of viral protein expression and RNA transcription. In addition, using specific siRNAs targeting ubiquitin reduces the production of viral progeny. In the presence of MG132 the staining for the envelope protein of DTMUV was dramatically reduced in comparison with the untreated control cells. Overall, our observations reveal an important role of the UPS in multiple steps of the DTMUV infection cycle and identify the UPS as a potential drug target to modulate the impact of DTMUV infection.

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