A novel gas liquid separator for direct sampling analysis of ultratrace arsenic in blood sample by hydride generation in-situ dielectric barrier discharge atomic fluorescence spectrometry

文献类型： 外文期刊

第一作者： Liu, Meitong

作者： Liu, Meitong;Liu, Tengpeng;Mao, Xuefei;Liu, Jixin;Qian, Yongzhong;Liu, Meitong;Liu, Tengpeng;Mao, Xuefei;Liu, Jixin;Qian, Yongzhong;Liu, Meitong;Ding, Lan;Liu, Jixin;Na, Xing

作者机构：

关键词： Arsenic; Blood; Direct sampling gas liquid separator; Dielectric barrier discharge; Gas phase enrichment; Atomic fluorescence spectrometry

期刊名称：TALANTA （ 影响因子：6.057； 五年影响因子：5.386 ）

ISSN： 0039-9140

年卷期： 2019 年 202 卷

页码：

收录情况： SCI

摘要： A novel direct sampling (DS-HG) system consisting of an enlarged gas liquid separator (GLS) coupled with a foam breaker was firstly utilized for the in-situ dielectric barrier discharge atomic fluorescence spectrometer (DBD-AFS). After direct dilution using 5% HCl (v:v), a prepared blood sample was introduced into the DS-HG with a UV digestion unit, of which arsenic hydrides directly generated from sample under 5% HCl (v:v) and 5 g/L KBH4 in 1.5 g/L. KOH. Herein, the newly designed DS-HG is capable of effectively eliminating foam generation deriving from protein in blood sample. Then, arsenic hydrides were trapped by 11 kV discharging at 110 mL/min air, and released by 13 kV at 180 mL/min H-2 orderly. Under the optimized conditions, the linearity ranged from 0.05 to 50 ng/mL with a regression coefficient (R-2) = 0.996. The method detection limit (LOD) was 7 pg arsenic (0.14 ng/mL), and relative standard deviation (RSD) of 10 repeated measurements for a real blood sample was 4.2%, indicating a good precision. The spiked recoveries for real samples were in the range of 97%-102%. Furthermore, arsenic presence in real blood samples measured by the proposed method were consistent (P > 0.05) with the microwave digestion ICP-MS. The whole analytical time can be controlled within 8 min including sample dilution. As a matter of fact, it is a favorable progress for DBD technique to eliminate matrix interferences of real samples based on the gas phase enrichment (GPE) principle, with advantages such as excellent sensitivity, digestion-free, fast and simple operation. Thus, the recommended DS-HG-in-situ DBD-AFS are suitable to the fast analysis of ultratrace arsenic in blood samples to protect human's health.

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