文献类型： 外文期刊
第一作者： Lu, Xing
作者： Lu, Xing;Tian, Juan;Wen, Hua;Jiang, Ming;Liu, Wei;Wu, Fan;Yu, Lijuan;Zhong, Shan;Zhong, Shan;Zhong, Shan
作者机构：
关键词： Microcystin; ZFL cell line; Transcriptome; Gene transcription; Signaling pathway
期刊名称：AQUATIC TOXICOLOGY （ 影响因子：4.964； 五年影响因子：5.071 ）
ISSN： 0166-445X
年卷期： 2019 年 212 卷
页码：
收录情况： SCI
摘要： Microcystin-LR (MC-LR) is a highly toxic hepatotoxin that poses great hazards to aquatic organisms and even human health. The zebrafish liver cell line (ZFL) is a valuable model for investigating toxicity and metabolism of toxicants. However, the toxicity of MC-LR and its effects on gene transcription of ZFL cells remains to be characterized. In this study, we determined the toxicity of MC-LR for ZFL cells and investigated the effects of MC-LR on cellular transcriptome dynamics. The EC50 of MC-LR for ZFL cells was 80.123 mu g/mL. The ZFL cells were exposed to 10 mu g/mL MC-LR for 0, 1, 3, 6, 12 or 24 h, and RNA-sequencing was performed to analyze gene transcription. A total of 10,209 genes were found to be regulated by MC-LR. The numbers of up- and down-regulated genes at different time points ranged from 2179 to 3202 and from 1501 to 2597, respectively. Furthermore, 1543 genes underwent differential splicing (AS) upon MC-LR exposure, of which 620 were not identified as differentially expressed gene (DEG). The effects of MC-LR on cellular functions were highly time-dependent. MAPK (mitogen-activated protein kinase) and FoxO (forkhead box O) signaling pathways were the most prominent pathways activated by MC-LR. Steroid biosynthesis and terpenoid backbone biosynthesis were the most enriched for the down-regulated genes. A gene regulatory network was constructed from the expression profile datasets and the candidate master transcription factors were identified. Our results shed light on the molecular mechanisms of MC-LR cellular toxicity and the transcriptome landscapes of ZFL cells upon MC-LR toxicity.
分类号：
- 相关文献
作者其他论文 更多>>
-
Effects of dietary hydroxy-cinnamic acid derivatives on growth, muscle, and intestinal parameters of Tilapia (Oreochromis niloticus)
作者：Li, Qing;Zhu, Shengqin;Liu, Juan;Li, Yanqing;Xue, Zhiyong;Fu, Min;Zhou, Zhigang;Yu, Lijuan;Fu, Min;Li, Yanqing;Zhou, Zhigang;Yu, Lijuan
关键词：Hydroxycinnamic acid derivatives; Tilapia; Growth; Muscle physical parameters; Intestinal morphology
-
LACCASE35 enhances lignification and resistance against Pseudomonas syringae pv. actinidiae infection in kiwifruit
作者：Li, Yawei;Zhang, Dongle;Wang, Xiaojie;Wu, Shunyuan;Liu, Pu;Wang, Xiaojie;Zhou, Rongrong;Fang, Zemin;Bai, Fuxi;Li, Rui;Liu, Wei;Huang, Lili
关键词：
-
Multi-scale structural influence of starch on their interaction of caffeic acid and starch after freeze-thaw: Taking potato starch and lotus seed starch as examples
作者：Zhao, Renjie;Li, Chi;Liu, Qiannan;Liu, Wei;Zhang, Liang;Zhang, Zhenzhen;Zhao, Ruixuan;Hu, Honghai;Yao, Jia;Li, Chi
关键词：Caffeic acid; Starch; Interaction
-
Influence of the 'painless' TRP channel on temperature-dependent escape and humidity-related pupation in Bactrocera dorsalis larvae
作者：Zhang, Yan;Zhang, Panpan;Luo, Zhicai;Wang, Qi;Zhang, Jie;Yang, Minghuan;Yan, Shanchun;Liu, Wei;Wang, Guirong
关键词：Bactrocera dorsalis; Bdorpainless; CRISPR/Cas9; extreme environments; escape behavior
-
Multienzyme cascade for synthesis of hydroxytyrosol via engineered Escherichia coli
作者：Xiong, Tianzhen;Li, Xinmeng;Yue, Huidie;Liu, Junling;Bai, Dingyuan;Li, Wei;Fan, Guangyan;Xiong, Tianzhen;Li, Xinmeng;Yue, Huidie;Liu, Junling;Bai, Dingyuan;Li, Wei;Fan, Guangyan;Xiong, Tianzhen;Liu, Wei;Xiong, Tianzhen
关键词：Hydroxytyrosol;
Escherichia coli ; Cascade reaction; Whole cell biotransformation -
Divergent Flowering Time Responses to Increasing Temperatures Are Associated With Transcriptome Plasticity and Epigenetic Modification Differences at FLC Promoter Region of Arabidopsis thaliana
作者：Han, Yu;Liu, Li;Lei, Mengyu;Si, Huan;Ji, Yan;Du, Qiao;Zhang, Wenjia;Zan, Yanjun;Han, Yu;Liu, Wei;Du, Qiao;Liu, Jianquan;Han, Yu;Liu, Wei;Du, Qiao;Liu, Jianquan;Zhu, Mingjia;Liu, Jianquan;Dai, Yifei
关键词：ecological adaptation; flowering time; gene co-expression network; plasticity
-
A wheat phytohormone atlas spanning major tissues across the entire life cycle provides novel insights into cytokinin and jasmonic acid interplay
作者：Yin, Huanran;Liu, Wei;Hu, Xin;Jia, Jingqi;Liu, Mengmeng;Wei, Jiaqi;Cheng, Yikeng;Gong, Xin;Li, Qiang;Yan, Wenhao;Chen, Wei;Jia, Jizeng;Gao, Lifeng;Fernie, Alisdair R.
关键词：wheat; phytohormones; metabolic regulatory network; DOF
