Molecular characterization of HLJ-073, a recombinant canine coronavirus strain from China with an ORF3abc deletion

文献类型： 外文期刊

第一作者： Chen, Si

作者： Chen, Si;Liu, Dafei;Tian, Jin;Kang, Hongtao;Guo, Dongchun;Jiang, Qian;Liu, Jiasen;Li, Zhijie;Hu, Xiaoliang;Qu, Liandong

作者机构：

关键词： Canine coronavirus; ORF3abc; Recombination; Pantropic; Viral cell tropism

期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.574； 五年影响因子：2.466 ）

ISSN： 0304-8608

年卷期： 2019 年 164 卷 8 期

页码：

收录情况： SCI

摘要： Canine enteric coronaviruses (CCoVs) are important enteric pathogens of dogs. CCoVs with different variations are typically pantropic and pathogenic in dogs. In this study, we isolated a CCoV, designated HLJ-073, from a dead 6-week-old male Pekingese with gross lesions and diarrhea. Interestingly, sequence analysis suggested that HLJ-073 contained a 350-nt deletion in ORF3abc compared with reference CCoV isolates, resulting in the loss of portions of ORF3a and ORF3c and the complete loss of ORF3b. Phylogenetic analysis based on the S gene showed that HLJ-073 was more closely related to members of the FCoV II cluster than to members of the CCoV I or CCoV II cluster. Furthermore, recombination analysis suggested that HLJ-073 originated from the recombination of FCoV 79-1683 and CCoV A76, which were both isolated in the United States. Cell tropism experiments suggested that HLJ-073 could effectively replicate in canine macrophages/monocytes and human THP-1 cells. This is the first report of the isolation of strain HLJ-073 in China, and this virus has biological characteristics that are different from those of other reported CCoVs.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序