Progress in Research and Prospects for Application of Precision Gene-Editing Technology Based on CRISPR-Cas9 in the Genetic Improvement of Sheep and Goats

文献类型： 外文期刊

第一作者： Lu, Zeyu

作者： Lu, Zeyu;Mu, Qing;Liu, Junyang;Chen, Yu;Wang, Haoyuan;Zhang, Yanjun;Su, Rui;Wang, Ruijun;Wang, Zhiying;Lv, Qi;Liu, Zhihong;Zhao, Yanhong;Lu, Zeyu;Mu, Qing;Liu, Junyang;Chen, Yu;Wang, Haoyuan;Zhang, Yanjun;Su, Rui;Wang, Ruijun;Wang, Zhiying;Lv, Qi;Liu, Zhihong;Zhao, Yanhong;Lu, Zeyu;Mu, Qing;Liu, Junyang;Chen, Yu;Wang, Haoyuan;Zhang, Yanjun;Su, Rui;Wang, Ruijun;Wang, Zhiying;Lv, Qi;Liu, Zhihong;Zhao, Yanhong;Zhang, Lingtian;Liu, Jiasen;Li, Yunhua

作者机构：

关键词： precision gene editing; CRISPR-Cas9; livestock genetic breeding; cashmere goat; editing efficiency; fixed point integration

期刊名称：AGRICULTURE-BASEL （ 影响因子：3.6； 五年影响因子：3.6 ）

ISSN：

年卷期： 2024 年 14 卷 3 期

页码：

收录情况： SCI

摘要： Due to recent innovations in gene editing technology, great progress has been made in livestock breeding, with researchers rearing gene-edited pigs, cattle, sheep, and other livestock. Gene-editing technology involves knocking in, knocking out, deleting, inhibiting, activating, or replacing specific bases of DNA or RNA sequences at the genome level for accurate modification, and such processes can edit genes at a fixed point without needing DNA templates. In recent years, although clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system-mediated gene-editing technology has been widely used in research into the genetic breeding of animals, the system's efficiency at inserting foreign genes is not high enough, and there are certain off-target effects; thus, it is not appropriate for use in the genome editing of large livestock such as cashmere goats. In this study, the development status, associated challenges, application prospects, and future prospects of CRISPR/Cas9-mediated precision gene-editing technology for use in livestock breeding were reviewed to provide a theoretical reference for livestock gene function analysis, genetic improvement, and livestock breeding that account for characteristics of local economies.

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