Anti-idiotypic nanobody-phage display-mediated real-time immuno-PCR for sensitive, simultaneous and quantitative detection of total aflatoxins and zearalenone in grains

文献类型： 外文期刊

第一作者： Ren, Xianfeng

作者： Ren, Xianfeng;Zhang, Qi;Yan, Tingting;Tang, Xiaoqian;Zhang, Wen;Yu, Li;Li, Peiwu;Wu, Wenhua;Ren, Xianfeng;Yan, Tingting;Zhang, Wen;Li, Peiwu;Zhang, Qi;Tang, Xiaoqian;Li, Peiwu;Yu, Li;Li, Peiwu;Li, Peiwu

作者机构：

关键词： Aflatoxin; Zearalenone; Nanobody; Phage display mediated immuno-polymerase chain reaction (PD-IPCR)

期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）

ISSN： 0308-8146

年卷期： 2019 年 297 卷

页码：

收录情况： SCI

摘要： An anti-idiotypic nanobody-phage display-mediated immuno-polymerase chain reaction (PD-IPCR) method was developed for simultaneous quantitative detection of total aflatoxins and zearalenone in cereals. Two phages, displaying the variable domain of the heavy chain anti-idiotypic nanobody that binds aflatoxin-or zearalenone-specific monoclonal antibody (1C11 or 2D3), were used as competitors for corresponding analytes. Specific DNA sequences encoding anti-idiotypic nanobodies were used to design the primers for PCR amplification. The results indicated that detection limits for total aflatoxins and zearalenone in a sample were 0.03 and 0.09 ng mL(-1), respectively. Recoveries of spiked aflatoxins and zearalenone were 80-118% and 76.7-111%, respectively. Validation results were in good agreement with the gold-standard high-performance liquid chromatography method. This report is the first to describe PD-IPCR for simultaneous quantitative detection of total aflatoxins and zearalenone in cereals.

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