ID3 mediates the TGF-beta 1-induced suppression of matrix metalloproteinase-1 in human granulosa cells

文献类型： 外文期刊

第一作者： Li, Hui

作者： Li, Hui;Shi, Zhendan;Li, Hui;Chang, Hsun-Ming;Leung, Peter C. K.;Li, Hui;Shi, Zhendan

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关键词： corpus luteum; extracellular matrix; ID3; matrix metalloproteinase-1; ovarian development; TGF-beta 1

期刊名称：FEBS JOURNAL （ 影响因子：5.542； 五年影响因子：5.5 ）

ISSN： 1742-464X

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收录情况： SCI

摘要： In the mammalian ovary, matrix metalloproteinase-1 (MMP1) is expressed in growing ovarian follicles, and MMP1-mediated extracellular matrix (ECM) remodeling plays a functional role in regulating the formation of corpus luteum. Transforming growth factor-beta 1 (TGF-beta 1) is an intraovarian growth factor that acts as a negative regulator of luteinization and progesterone production in human granulosa-lutein (hGL) cells. At present, whether TGF-beta 1 regulates the expression of MMP1 and thus affects ECM remodeling during corpus luteum formation remains largely unknown. The aim of this study was to investigate the effects of TGF-beta 1 and the molecular mechanisms by which it regulates the expression of MMP1 in immortalized human granulosa cells lines (SVOG) and primary hGL cells (obtained from consenting patients undergoing IVF treatment). We used inhibition approaches including a competitive antagonist for endogenous TGF-beta type II receptor, pharmacological inhibitors (SB431542 and dorsomorphin), and specific small interfering RNA-targeted knockdown of ALK5 type I receptor and SMAD4 to demonstrate that TGF-beta 1 downregulates the expression and production of MMP1 via a T beta RII/ALK5-mediated SMAD-dependent signaling pathway in hGL cells. Additionally, our results show that the suppressive effect of TGF-beta 1 on the expression of MMP1 is mediated by a transcription factor, the inhibitor of differentiation 3 (ID3) protein. Our findings provide insights into the molecular interactions and mechanisms of TGF-beta 1 and ID3 during the regulation of MMP1 in hGL cells.

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