Isolation and characterization of AHL-degrading bacteria from fish and pond sediment

文献类型： 外文期刊

第一作者： Li Miaoyu

作者： Li Miaoyu;Xie Jun;Li Miaoyu;Xi Bingwen;Qin Ting;Chen Kai;Xie Jun

作者机构：

关键词： quorum sensing (QS); Aeromonas hydrophila; probiotic; Enterobacter; Staphylococcus

期刊名称：JOURNAL OF OCEANOLOGY AND LIMNOLOGY （ 影响因子：1.265； 五年影响因子：1.276 ）

ISSN： 2096-5508

年卷期： 2019 年 37 卷 4 期

页码：

收录情况： SCI

摘要： Quorum sensing (QS) disruption is considered as a potential alternative strategy to combat bacterial diseases in aquaculture. In this study, we isolated and identified bacteria degrading QS molecules from pond sediment and fish intestine. A total of 132 strains were obtained in the enrichment culture, of which two strains were identified as Enterobacter sp. f003 and Staphylococcus sp. sw120, being isolated from the fish intestine and pond sediment, respectively. We found that strains f003 and sw120 could degrade acyl-homoserine lactones (AHLs) and cause no hemolysis of sheep red blood cells. The AHL lactonase (aiiA) homologous gene in the two strains was detected in PCR amplification and the high-degrading activity to N-hexanoyl-L-homoserine lactone (C6-HSL) and AHLs secreted from pathogenic Aeromonas hydrophila was assessed. Meanwhile, the artificial infection of cyprinid Carassius auratus gibelio with intraperitoneal injection showed that the two strains were avirulent. Therefore, the obtained indigenous bacteria are candidate probiotics against pathogenic A. hydrophila in aquaculture.

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