iTRAQ protein profile analysis of young and old leaves of cotton (Gossypium hirsutum L.) differing in response to alkali stress

文献类型： 外文期刊

第一作者： Zhong, Xiuli

作者： Zhong, Xiuli;Gu, Fengxue;Liu, Qi;Li, Haoru;Guo, Rui;Hu, Yongjun;Zhao, Long;Zhou, Ji

作者机构：

关键词： Gossypium hirsutum L; Young leaves; Old leaves; iTRAQ; Proteomics; Alkali stress

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：4.27； 五年影响因子：4.816 ）

ISSN： 0981-9428

年卷期： 2019 年 141 卷

页码：

收录情况： SCI

摘要： Proteins will provide a new perspective and deeper understanding for the research of crop alkali tolerance. The aims of this study were to determine and identify the differentially abundant proteins and adaptive mechanisms to alkali tolerance between young and old leaves of cotton. The 4704 proteins were identified, in which 1490 were significantly changed in young leaves and 563 were changed in old leaves in response to alkali stress. The differentially abundant proteins were classified into 10 functional categories in the young leaves, and only 3 functional categories were involved in the old leaves. In the photoreaction system, the accumulations of differential proteins, especially Psb proteins, were higher in young leaves than in old leaves. Compared with old leaves, the carbon metabolism was enhanced significantly through an increased chlorophyll content and increased expression of key proteins for carbon metabolism in young leaves. Furthermore, alkali stress revealed more complex effects on the nitrogen metabolism in young leaves than that in old leaves. Our results demonstrated that during adaptation of cotton to alkali stress, young and old leaves have distinct mechanisms of molecular metabolism regulation. The metabolic flexibility was more remarkable in young leaves than in old leaves; therefore, the alkali tolerance of young leaves is more efficient. These data will increase our understanding of alkali-tolerant mechanisms in higher plants.

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