A high density SLAF-seq SNP genetic map and QTL for seed size, oil and protein content in upland cotton

文献类型： 外文期刊

第一作者： Cai, Xiaoyan

作者： Cai, Xiaoyan;Liu, Fang;Wang, Wenwen;Sun, Ying;Yang, Peng;Yang, Le;Ma, Junrui;Ou, Yuncan;Liu, Tianpeng;Ali, Iftikhar;Liu, Dajun;Zhang, Jian;Teng, Zhonghua;Guo, Kai;Liu, Dexin;Zhang, Zhengsheng

作者机构：

关键词： Upland cotton; SLAF-seq; Seed size; Oil; Protein; QTL

期刊名称：BMC GENOMICS （ 影响因子：3.969； 五年影响因子：4.478 ）

ISSN： 1471-2164

年卷期： 2019 年 20 卷

页码：

收录情况： SCI

摘要： BackgroundCotton is a leading natural fiber crop. Beyond its fiber, cottonseed is a valuable source of plant protein and oil. Due to the much higher value of cotton fiber, there is less consideration of cottonseed quality despite its potential value. Though some QTL controlling cottonseed quality have been identified, few of them that warrant further study are known. Identifying stable QTL controlling seed size, oil and protein content is necessary for improvement of cottonseed quality.ResultsIn this study, a recombinant inbred line (RIL) population was developed from a cross between upland cotton cultivars/lines Yumian 1 and M11. Specific locus amplified fragment sequencing (SLAF-seq) technology was used to construct a genetic map that covered 3353.15cM with an average distance between consecutive markers of 0.48cM. The seed index, together with kernel size, oil and protein content were further used to identify QTL. In total, 58 QTL associated with six traits were detected, including 13 stable QTL detected in all three environments and 11 in two environments.ConclusionA high resolution genetic map including 7033 SNP loci was constructed through specific locus amplified fragment sequencing technology. A total of 13 stable QTL associated with six cottonseed quality traits were detected. These stable QTL have the potential for fine mapping, identifying candidate genes, elaborating molecular mechanisms of cottonseed development, and application in cotton breeding programs.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序