Fengycin produced by Bacillus subtilis NCD-2 is involved in suppression of clubroot on Chinese cabbage

文献类型： 外文期刊

第一作者： Guo, Song

作者： Guo, Song;Zhang, Jun-wei;Li, Xu;Asif, Muhammad;Jiang, Wen-jun;Zhang, Li-qun;Guo, Song;Zhang, Jun-wei;Li, Xu;Asif, Muhammad;Jiang, Wen-jun;Zhang, Li-qun;Dong, Li-hong;Guo, Qing-gang;Ma, Ping

作者机构：

关键词： Plasmodiophora brassicae; Clubroot; Fengycin; Bacillus; qPCR

期刊名称：BIOLOGICAL CONTROL （ 影响因子：3.687； 五年影响因子：3.962 ）

ISSN： 1049-9644

年卷期： 2019 年 136 卷

页码：

收录情况： SCI

摘要： Clubroot, caused by the obligate parasite Plasmodiophora brassicae, is one of the most important diseases of Brassicaceae family. Bacillus spp. that produce lipopeptide antibiotics are often used as biocontrol agents to suppress soilborne diseases. B. subtilis NCD-2 isolated from the cotton rhizosphere has been found to inhibit pathogenic fungi through the production of fengycin-type cyclopeptides. In this study, the biocontrol efficacy of B. subtilis NCD-2 against P. brassicae and the role of fengycin in disease suppression were evaluated on Chinese cabbage in glasshouse. B. subtilis NCD-2 reduced clubroot disease incidence and severity up to 47.7% and 51.6%, respectively, while the fengycin defective mutant Delta fenC and a PhoP/PhoR two-component regulatory system mutant, Delta phoP, were unable to prevent this disease. In vitro, P. brassicae resting spore cells were lysed when treated with standard fengycin and crude fengycin extracts of the wild type B. subtilis NCD-2, but not of its mutant Delta fenC. Moreover, the population dynamics of B. subtilis and P. brassicae was determined using quantitative real-time PCR (qPCR). The results were surprising that the wild type B. subtilis NCD-2 and its mutant Delta fenC colonized at similar rates in the rhizosphere, while levels of P. brassicae DNA in the rhizosphere and root tissue were reduced following B. subtilis NCD-2 treatment, but there was no effect of the mutant Delta fenC. Thus, the production of fengycins in B. subtilis NCD-2 may play a promising role in the suppression of P. brassicae in the rhizosphere and reduction of incidence of clubroot disease in Chinese cabbage.

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