Antimicrobial and antibiofilm efficacy and mechanism of essential oil from Citrus Changshan-huyou Y. B. chang against Listeria monocytogenes

文献类型： 外文期刊

第一作者： Li, Gaoyang

作者： Li, Gaoyang;Fu, Fuhua;Shan, Yang;Guo, Jiajing;Li, Gaoyang;Fu, Fuhua;Liang, Zengenni;Shan, Yang;Gao, Zhipeng;Zhu, Hui

作者机构：

关键词： Citrus changshan-huyou YB chang; Essential oil; Listeria monocytogenes; Antimicrobial; Antibiofilm; Transcriptome

期刊名称：FOOD CONTROL （ 影响因子：5.548； 五年影响因子：5.498 ）

ISSN： 0956-7135

年卷期： 2019 年 105 卷

页码：

收录情况： SCI

摘要： Listeria monocytogenes (L. monocytogenes) is an important foodborne pathogen which may cause listeriosis and poses potential risks to human health. The aim of this study was to estimate the efficacy of essential oil from Citrus Changshan-huyou Y.B. Chang (Huyou) on both planktonic cells and biofilm against L. monocytogenes, and further to elucidate the potential mechanism of this action. Results showed that Citrus Changshan-huyou Y.B. Chang essential oil (Huyou EO) exhibited significantly in vitro antimicrobial ability against L. monocytogenes, both of scanning electron microscopy (SEM) and transmission electron microscopy (TEM) observations confirmed the physical damage, considerable morphological alteration and the leakage of intracellular constituents. Meanwhile, XTT and crystal violet (CV) assay indicated that Huyou EO was able to destroy preformed biofilm through decreasing the biomass and cellular activity. SEM, confocal scanning laser microscopy (CLSM) and COMSTAT analysis revealed the extensive disruption and architectural changes of biofilm. Moreover, the potential antimicrobial mechanisms were demonstrated by RNA sequencing. Enriched GO analysis identified 8 terms related to cellular components (CC), 50 terms for biological processes (BP) and 21 terms for molecular functions (MF). 35 pathways were significantly enriched in KEGG analysis. The transcriptome analysis offered novel antimicrobial molecular mechanisms of Huyou EO.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序