An efficient Agrobacterium-mediated transformation system of Platycodon grandiflorum

文献类型： 外文期刊

第一作者： Du, Xiaoyu

作者： Du, Xiaoyu;Yuan, Yuan;Du, Xiaoyu;Fu, Qinghui;Liu, Yunjun;Jiang, Zhennan;Fu, Qinghui;Yuan, Yuan

作者机构：

关键词： Platycodon grandiflorum; Transformation; Agrobacterium tumefaciens; Agrobacterium rhizogenes; Morphogenic regulator; Gene editing

期刊名称：PLANT CELL TISSUE AND ORGAN CULTURE （ 影响因子：2.4； 五年影响因子：2.6 ）

ISSN： 0167-6857

年卷期： 2025 年 161 卷 2 期

页码：

收录情况： SCI

摘要： Platycodon grandiflorum is a globally cultivated ornamental flower as well as a widely utilized traditional medicinal plant and food ingredient. Despite its significance, genetic transformation efforts in P. grandiflorum have been largely confined to the production of transgenic hairy roots. In this study, we established and optimized genetic transformation protocols for P. grandiflorum. We optimized transformation conditions using A. tumefaciens strain EHA105 carrying the pCambia3301 plasmid, achieving 6.77% efficiency with leaf explants. Moreover, we developed a transformation system mediated by Agrobacterium rhizogenes, utilizing the ternary vector p3301WusBBM, which contains the morphogenic regulator genes Wus2 and ZmBBM2. This approach significantly enhanced hairy root induction and plant regeneration efficiencies to 43.75% and 21.88%, respectively, thereby improving regeneration efficiency, which has been a challenge in P. grandiflorum transformation. Furthermore, with the improved A. rhizogenes-mediated transformation system combined with CRISPR/Cas9 technology, we achieved precise editing of the endogenous P. grandiflorum gene chr2.2745, attaining a genome editing efficiency of 16.70%. This study establishes an efficient platform for genetic transformation and genome editing in P. grandiflorum, enabling advanced functional genomics research and germplasm enhancement.

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