Recovery of a hypolipidemic polysaccharide from artificially cultivated Sanghuangporus vaninii with an effective method

文献类型： 外文期刊

第一作者： Zhang, Zuo-fa

作者： Zhang, Zuo-fa;Song, Ting-ting;Chen, Jian-fei;Lv, Guo-ying

作者机构：

关键词： Sanghuangporus vaninii; hypolipidemic polysaccharide; acid-ethanol pretreatment; zebrafish model; structure illustration

期刊名称：FRONTIERS IN NUTRITION （ 影响因子：5.0； 五年影响因子：5.7 ）

ISSN： 2296-861X

年卷期： 2023 年 9 卷

页码：

收录情况： SCI

摘要： In this study, an effective method was developed to extract the polysaccharide from Sanghuangporus vaninii (PFSV) by destroying the cell wall. Box-Behnken design was employed to determine the optimal processing conditions as follows: processing temperature (80 degrees C), processing time (0.81 h) and amount of HCl (1.5 ml). Under these conditions, the yield of PFSV reached 5.94 +/- 0.16%. The purified polysaccharide (PFSV-2) was found to be a hetero-polysaccharide with an average molecular weight of 20.377 kDa. The backbone of PFSV-2 was composed of an -> 6)-alpha-Galp-(1 -> and -> 2,6)-beta-Manp-(1 -> and -> 2)-alpha-Fucp-(1 -> and was branched of t-alpha-Manp-(1 -> at position 2 of residue B. PFSV-2 showed hypolipidemic activity by decreasing lipid accumulation and the levels of total cholesterol and triglycerides in zebrafish larvae. Furthermore, PFSV-2 downregulated the pparg, fasn, and HMGCRb genes and upregulated the pparab and acaca genes. These findings suggested PFSV-2 may be a promising candidate in lipid regulation therapy.

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