Nitrogen fertilization modulates beneficial rhizosphere interactions through signaling effect of nitric oxide

文献类型： 外文期刊

第一作者： Kang, An

作者： Kang, An;Zhang, Nan;Xun, Weibing;Dong, Xiaoyan;Xiao, Ming;Liu, Zihao;Xu, Zhihui;Feng, Haichao;Zou, Jianwen;Shen, Qirong;Dong, Xiaoyan;Zou, Jianwen;Zhang, Ruifu

作者机构：

期刊名称：PLANT PHYSIOLOGY （ 影响因子：8.005； 五年影响因子：9.115 ）

ISSN： 0032-0889

年卷期： 2022 年 188 卷 2 期

页码：

收录情况： SCI

摘要： Chemical nitrogen (N) fertilization is customary for increasing N inputs in agroecosystems. The nutritional effects of N fertilization on plants and soil microbes have been well studied. However, the signaling effects of N fertilization on rhizosphere plant-microbe interactions and the following feedback to plant performance remain unknown. Here, we investigated the effect of different N fertilizations on the behavior of the plant growth-promoting rhizobacteria (PGPR) Bacillus velezensis SQR9 in the cucumber (Cucumis sativus L.) rhizosphere. Moderate N fertilization promoted higher rhizosphere colonization of strain SQR9 than insufficient or excessive N input. Nitric oxide (NO) produced through the denitrification process under N fertilization was identified as the signaling molecule that dominates the root colonization of PGPR, and this effect could be neutralized by the NO-specific scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxy-3-oxide. Gene expression analysis demonstrated that NO regulated the biofilm formation of strain SQR9 by affecting the synthesis of extracellular matrix gamma-polyglutamic acid, consequently impacting its root colonization. Finally, we demonstrated that moderate N fertilization-modulated enhanced PGPR root colonization can significantly promote plant growth and nitrogen use efficiency. This study provides insights into our understanding of the beneficial rhizosphere plant-microbe interactions under N fertilization and suggests that rational fertilization is critical to promote beneficial rhizosphere interactions for sustainable agricultural production. Moderate N fertilization enhances plant-beneficial rhizobacteria interactions and promotes plant N use efficiency, which is mediated by the signaling effect of denitrification-produced NO.

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