文献类型： 外文期刊
第一作者： Jia, Wei
作者： Jia, Wei;Wang, Wenhan;Liu, Peng;Zhang, Meiyan;Li, Qiaozhen;Zhang, Henan;Zhang, Jingsong;Jia, Wei;Yu, Yanzhen;Wang, Huimin;Zhang, Hongtao;Li, Huaxiang
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期刊名称：SEPARATIONS （ 影响因子：2.7； 五年影响因子：2.7 ）
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年卷期： 2024 年 11 卷 11 期
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收录情况： SCI
摘要： The structure and characteristics of LEOPs, beta-oligosaccharides from the fruiting body of Lentinus edodes obtained via acid degradation and gel permeation chromatography, were investigated. We performed high-performance liquid chromatography, infrared spectroscopy, methylation analysis, nuclear magnetic resonance, and correlated activity experiments, including antioxidant, immunomodulatory, and liver injury protection to gain insights. LEOPs comprised an oligosaccharide (Mw 2445 Da) based on six beta-1, 3-D-glucose residues as the main chain and six beta-1, 6-D-glucose residues as the side chain. Surface plasmon resonance analysis indicated that LEOPs directly bound to dectin-1, which facilitated their immunoenhancing activity via downstream NF-kappa B activation. The results implied that LEOPs may be the active unit of the shiitake beta-glucan. The determination of LEOPs structure was performed to reveal the anti-tumor effect and immune-regulatory function of shiitake beta-glucan on a molecular level to provide a basis.
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