A novel idiotypic-based noncompetitive immunoassay based on the dissociation of immunocomplex for detection atrazine in vegetables

文献类型： 外文期刊

第一作者： Zhang, Xing

作者： Zhang, Xing;Liu, Beibei;Zhai, Rongqi;Liu, Guangyang;Chen, Linglong;Liu, Junjiang;Xu, Donghui;Chen, Ge;Liu, Beibei;Jin, Maojun;Wang, Jing

作者机构：

关键词： Noncompetitive immunoassay; Low-molecular-weight contaminant; Immunocomplex; Anti-idiotypic antibody; Atrazine

期刊名称：FOOD CHEMISTRY （ 影响因子：9.8； 五年影响因子：9.7 ）

ISSN： 0308-8146

年卷期： 2025 年 486 卷

页码：

收录情况： SCI

摘要： Atrazine is a selective systemic herbicide of the chlorotriazine class that is commonly used for controlling annual broadleaf and grassy weeds. However, its residue can harm the environment and human health. We have therefore developed a novel idiotypic-based noncompetitive immunoassay for the detection of atrazine by exploiting the distinctive recognition properties of anti-idiotypic antibodies. This method utilizes atrazine as a "key" to specifically dissociation the "lock" of an anti-idiotypic antibody and detection antibody immunocomplex, thereby enabling both qualitative and quantitative analysis of atrazine. Following all necessary optimizations, this method was capable exhibiting a limit of detection (LOD) of 0.52 ng/mL with R2 = 0.990, and a linear range of 1.76 to 2604.61 ng/mL. This immunoassay offered its potential towards detection of atrazine in water and 10 kinds of vegetable samples with a recovery ranging from 70.76 to 119.28 %. The idiotypic-based noncompetitive immunoassay provides a well suitable method for low-molecular-weight contaminants immunoassay.

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