Genomics and proteomics of Apis mellifera filamentous virus isolated from honeybees in China

文献类型： 外文期刊

第一作者： Yang, Dahe

作者： Yang, Dahe;Diao, Qingyun;Yang, Dahe;Wang, Jun;Wang, Xi;Deng, Fei;Wang, Manli;Hu, Zhihong;Yang, Dahe;Wang, Jun;Wang, Xi;Deng, Fei;Wang, Manli;Hu, Zhihong;Hou, Chunsheng

作者机构：

关键词： Apis mellifera filamentous Virus (AmFV); per os infectivity factor 4 (PIF4); Genome sequence; Proteomics; Structural proteins; Naldaviricetes

期刊名称：VIROLOGICA SINICA （ 影响因子：6.947； 五年影响因子：5.997 ）

ISSN： 1674-0769

年卷期： 2022 年 37 卷 4 期

页码：

收录情况： SCI

摘要： Apis mellifera filamentous virus (AmFV) is a large DNA virus that is endemic in honeybee colonies. The genome sequence of the AmFV Swiss isolate (AmFV CH-C05) has been reported, but so far very few molecular studies have been conducted on this virus. In this study, we isolated and purified AmFV (AmFV CN) from Chinese honeybee (Apis mellifera) colonies and elucidated its genomics and proteomics. Electron microscopy showed ovoid purified virions with dimensions of 300-500 x 210-285 nm, wrapping a 3165 x 40 nm filamentous nucleocapsid in three figure-eight loops. Unlike AmFV CH-C05, which was reported to have a circular genome, our data suggest that AmFV CN has a linear genome of approximately 493 kb. A total of 197 ORFs were identified, among which 36 putative genes including 18 baculoviral homologs were annotated. The overall nucleotide similarity between the CN and CH-C05 isolates was 96.9%. Several ORFs were newly annotated in AmFV CN, including homologs of per os infectivity factor 4 (PIF4) and a putative integrase. Phylogenomic analysis placed AmFVs on a separate branch within the newly proposed virus class Naldaviricetes. Proteomic analysis revealed 47 AmFV virion-associated proteins, of which 14 had over 50% sequence coverage, suggesting that they are likely to be main structural proteins. In addition, all six of the annotated PIFs (PIF-0-5) were identified by proteomics, suggesting that they may function as entry factors in AmFV infection. This study provides fundamental information regarding the molecular biology of AmFV.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序