A novel recombinant adenovirus expressing apoptin and melittin genes kills hepatocellular carcinoma cells and inhibits the growth of ectopic tumor

文献类型： 外文期刊

第一作者： Wu, Jingqiao

作者： Wu, Jingqiao;Lan, Zhaoyu;Li, Xin;He, Jinling;Zhang, Dongchao;Jin, Tianming;Jin, Tianming;Wu, Jingqiao;Lan, Zhaoyu;Li, Xin;He, Jinling;Zhang, Dongchao;Zhang, Dongchao;Jin, Tianming

作者机构：

关键词： Recombinant adenovirus; Melittin; Apoptin; Hepatocellular carcinoma

期刊名称：INVESTIGATIONAL NEW DRUGS （ 影响因子：3.0； 五年影响因子：2.9 ）

ISSN： 0167-6997

年卷期： 2024 年 42 卷 4 期

页码：

收录情况： SCI

摘要： HCC is the most common fatal malignancy. Although surgical resection is the primary treatment strategy, most patients are not eligible for resection due to tumor heterogeneity, underlying liver disease, or comorbidities. Therefore, this study explores the possibility of multi-molecular targeted drug delivery in treating HCC. In this study, we constructed the recombinant adenovirus co-expressing apoptin and melittin (MEL) genes. The inhibitory effect of the recombinant adenovirus on hepatocellular carcinoma cells was detected through experiments on cell apoptosis, migration, invasion, and other factors. The tumor inhibitory effect in vivo was assessed using subcutaneous HCC mice. Results showed that recombinant adenovirus co-expressing anti-tumor genes TAT and apoptin, RGD and MEL can significantly inhibit the proliferation, migration, and invasion of HCC cells by inducing an increase in reactive oxygen species (ROS) levels, upregulation of apoptotic proteins such as Bax, cleaved caspase-3, and cleaved caspase-9, and downregulation of the anti-apoptotic protein Bcl-2. In subcutaneous HCC mice, recombinant adenovirus induced significant apoptosis in tumor, and inhibited tumor growth. In conclusion, recombinant adenovirus co-expressing apoptin and MEL can inhibit the growth and proliferation of tumor cells both in vivo and in vitro.

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