Graphene enhances artemisinin production in the traditional medicinal plant Artemisia annua via dynamic physiological processes and miRNA regulation

文献类型： 外文期刊

第一作者： Cao, Junfeng

作者： Cao, Junfeng;Rahman, Saeed ur;Qin, Wei;Wang, Yuliang;Tang, Kexuan;Chen, Zhiwen;Liu, Zehui;Qiao, Jun;Zhao, Jianguo;Chen, Zhiwen;Lin, Jialing;Hou, Lipan;Huang, Chaochen;Wen, Tingting;Li, Chenyi;Wang, Luyao;Zhao, Yongyan;Wang, Luyao;Zhao, Yongyan;Yan, Ning;Wang, Jie;Si, Tong;Shi, Yannan

作者机构： Shanghai Jiao Tong Univ, Fudan SJTU Nottingham Plant Biotechnol R&D Ctr, Joint Int Res Lab Metab & Dev Sci, Frontiers Sci Ctr Transformat Mol,Sch Agr & Biol,, Shanghai 200240, Peoples R China;Shanxi Datong Univ, Engn Res Ctr Coal Based Ecol Carbon Sequestrat Tec, Minist Educ, Key Lab Natl Forest & Grass Adm Applicat Graphene, Datong 037009, Peoples R China;Chinese Acad Sci, CAS Ctr Excellence Mol Plant Sci, Inst Plant Physiol & Ecol, Natl Key Lab Plant Mol Genet, Shanghai 200032, Peoples R China;Zhejiang Univ, Hainan Inst, Yazhou Bay Sci & Technol City, Yongyou Ind Pk, Sanya 572000, Peoples R China;Zhejiang Univ, Coll Agr & Biotechnol, Hangzhou 310058, Peoples R China;Qingdao Agr Univ, Coll Agron, Shandong Prov Key Lab Dryland Farming Technol, Qingdao 266109, Peoples R China;Hebei Acad Agr & Forestry Sci, Hebei Branch, Inst Millet Crops, China Natl Sorghum Improvement Ctr, Shijiazhuang 050035, Peoples R China

关键词： Artemisia annua; artemisinin; glandular secreting trichomes GSTs; miRNA; graphene

期刊名称：PLANT COMMUNICATIONS （ 2022影响因子：10.5； 五年影响因子：10.5 ）

ISSN： 2590-3462

年卷期： 2024 年 5 卷 3 期

页码：

收录情况： SCI

摘要： We investigated the effects of graphene on the model herb Artemisia annua, which is renowned for producing artemisinin, a widely used pharmacological compound. Seedling growth and biomass were promoted when A. annua was cultivated with low concentrations of graphene, an effect which was attributed to a 1.4 -fold increase in nitrogen uptake, a 15%-22% increase in chlorophyll fluorescence, and greater abundance of carbon cycling-related bacteria. Exposure to 10 or 20 mg/L graphene resulted in a -60% increase in H2O2, and graphene could act as a catalyst accelerator, leading to a 9 -fold increase in catalase (CAT) activity in vitro and thereby maintaining reactive oxygen species (ROS) homeostasis. Importantly, graphene exposure led to an 80% increase in the density of glandular secreting trichomes (GSTs), in which artemisinin is biosynthesized and stored. This contributed to a 5% increase in artemisinin content in mature leaves. Interestingly, expression of miR828 was reduced by both graphene and H2O2 treatments, resulting in induction of its target gene AaMYB17, a positive regulator of GST initiation. Subsequent molecular and genetic assays showed that graphene-induced H2O2 inhibits micro -RNA (miRNA) biogenesis through Dicers and regulates the miR828-AaMYB17 module, thus affecting GST density. Our results suggest that graphene may contribute to yield improvement in A. annua via dynamic physiological processes together with miRNA regulation, and it may thus represent a new cultivation strategy for increasing yield capacity through nanobiotechnology.

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