文献类型： 外文期刊
第一作者： Zhai, Yutong
作者： Zhai, Yutong;Yu, Yonghao;Xu, Pengfei;Zeng, Xianru;Long, Xiuzhen;Wei, Dewei;He, Zhan;Gao, Xuyuan;Zhai, Yutong;Yu, Yonghao;Xu, Pengfei;Zeng, Xianru;Long, Xiuzhen;Wei, Dewei;He, Zhan;Gao, Xuyuan;Zhai, Yutong;Yu, Yonghao;Xu, Pengfei;Zeng, Xianru;Long, Xiuzhen;Wei, Dewei;He, Zhan;Gao, Xuyuan
作者机构：
关键词： reference genes; RT-qPCR; Bactrocera tau; different stages; different body parts
期刊名称：INSECTS （ 影响因子：2.9； 五年影响因子：3.3 ）
ISSN：
年卷期： 2025 年 16 卷 5 期
页码：
收录情况： SCI
摘要： The selection of appropriate reference genes is critical for standardizing quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) data, thereby ensuring accurate and reliable results of gene expression analysis. In this study, we identified 10 candidate reference genes (encoding alpha-tubulin, G6PDH, Rab1, RT, RPS13, beta-tubulin, DPH1, HSP90, GAPDH, and CP) and evaluated their suitability for use as reference genes in the pest insect, Bactrocera tau. Analysis was conducted using three software-based methods-Delta CT, NormFinder, and BestKeeper-alongside the online tool RefFinder. Expression levels of these genes were analyzed across various B. tau developmental stages and body parts. The overall ranking of reference gene stability scores was as follows: alpha-tubulin > G6PDH > CP > beta-tubulin > RT > HSP90 > GAPDH > DPH1 > RPS13 > Rab1. Ultimately, alpha-tubulin and G6PDH were identified as the most stable reference genes for B. tau.
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