Indole metabolism mechanisms in a new, efficient indole-degrading facultative anaerobe isolate Enterococcus hirae GDIAS-5

文献类型： 外文期刊

第一作者： Deng, Jun-Jin

作者： Deng, Jun-Jin;Deng, Dun;Chen, Hong-Ping;Ma, Xian-Yong;Li, Jia-Zhou;Wang, Zhi-Lin;Luo, Xiao-Chun;Liu, Shu-Yang

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关键词： Indole degradation; Enterococcus hirae; Anaerobic degradation; Iron metabolism; Indole oxygenase

期刊名称：JOURNAL OF HAZARDOUS MATERIALS （ 影响因子：14.224； 五年影响因子：12.984 ）

ISSN： 0304-3894

年卷期： 2022 年 434 卷

页码：

收录情况： SCI

摘要： Indole is an inter-species and inter-kingdom signaling molecule widespread in the natural world. A large amount of indole in livestock wastes makes it difficult to be degraded, which causes serious malodor. Identifying efficient and eco-friendly ways to eliminate it is an urgent task for the sustainable development of husbandry. While bioconversion is a widely accepted means, the mechanism of indole microbial degradation is little understood, especially under anaerobic conditions. Herein, a new Enterococcus hirae isolate GDIAS-5, effectively degraded 100 mg/L indole within 28 h aerobically or 5 days anaerobically. Three intermediates (oxindole, isatin, and catechol) were identified in indole degradation, and catechol was further degraded by a meta-cleavage catabolic pathway. Two important processes for GDIAS-5 indole utilization were discovered. One is Fe(III) uptake and reduction, which may be a critical process that is coupled with indole oxidation, and the other is the entire pathway directly involved in indole oxidation and metabolism. Furthermore, monooxygenase ycnE responsible for indole oxidation via the indoleoxindole-isatin pathway was identified for the first time. Bioinformatic analyses showed that ycnE from E. hirae formed a phylogenetically separate branch from monooxygenases of other species. These findings provide new targets and strategies for synthetic biological reconstruction of indole-degrading bacteria.

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