Application of Biotechniques for In Vitro Virus and Viroid Elimination in Pome Fruit Crops

文献类型： 外文期刊

第一作者： Bettoni, Jean Carlos

作者： Bettoni, Jean Carlos;Wang, Min-Rui;Li, Jing-Wei;Fan, Xudong;Fazio, Gennaro;Hurtado-Gonzales, Oscar P.;Volk, Gayle M.;Wang, Qiao-Chun

作者机构：

关键词： chemotherapy; cryotherapy; micrografting; shoot tip culture; thermotherapy; virus and viroid detection

期刊名称：PHYTOPATHOLOGY （ 影响因子：3.2； 五年影响因子：3.9 ）

ISSN： 0031-949X

年卷期： 2024 年

页码：

收录情况： SCI

摘要： Sustainable production of pome fruit crops is dependent upon having virus-free planting materials. The production and distribution of plants derived from virus- and viroid-negative sources is necessary not only to control pome fruit viral diseases but also for sustainable breeding activities, as well as the safe movement of plant materials across borders. With variable success rates, different in vitro-based techniques, including shoot tip culture, micrografting, thermotherapy, chemotherapy, and shoot tip cryotherapy, have been employed to eliminate viruses from pome fruits. Higher pathogen eradication efficiencies have been achieved by combining two or more of these techniques. An accurate diagnosis that confirms complete viral elimination is crucial for developing effective management strategies. In recent years, considerable efforts have resulted in new reliable and efficient virus detection methods. This comprehensive review documents the development and recent advances in biotechnological methods that produce healthy pome fruit plants.

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