Genome-wide analysis of fatty acid desaturase (FAD) gene family in Camellia sinensis: Identification, expression and their biochemical functions in low temperature resistance

文献类型： 外文期刊

第一作者： Jin, Ya

作者： Jin, Ya;Wang, Yingqi;Jin, Ya;Lu, Qinhua;Ren, Ning;Shen, Guoxin;Li, Da;Li, Qingsheng;Liu, Liping

作者机构：

关键词： Camellia sinensis; FAD gene family; Sphingolipid Delta 8 desaturase; Low temperature

期刊名称：INDUSTRIAL CROPS AND PRODUCTS （ 影响因子：6.2； 五年影响因子：6.2 ）

ISSN： 0926-6690

年卷期： 2024 年 222 卷

页码：

收录情况： SCI

摘要： Fatty acid desaturase (FAD) is critical for unsaturated fatty acids (UFAs) biosynthesis and plays crucial roles in plants' response to low temperature. Nevertheless, a comprehensive genomic analysis of the FAD gene family in Camellia sinensis remain unreported. This study employed bioinformatics to identify 17 CsFAD genes, which are unequally distributed across 9 chromosomes. CsFAD proteins are localized to chloroplasts and/or endoplasmic reticulum (ER) by subcellular localization predictions. Phylogenetic categorization divides CsFADs into six subfamilies. The cis-elements are related to hormonal regulation and adversity response. Additionally, differential expression patterns of CsFADs across various tissues were observed. qRT-PCR analysis revealed a marked upregulation in the expression of CsSLD3 and CsSLD4 (encoding sphingolipid Delta 8 desaturase) under cold stress conditions. Moreover, subcellular localization assays in tobacco leaves confirmed the presence of CsSLD3 and CsSLD4 within ER. Yeast heterologous expression demonstrated the capacity of CsSLD3/4 to transform ceramide (t18:0) into ceramide(t18:1), and overexpression of CsSLD3/4 enhanced yeast tolerance to low temperature. Thus, this comprehensive analysis of the CsFAD gene family furnishes a robust foundation for future study into the biological functions of CsFAD genes, and offers a profound insight into their regulatory roles in stress resilience.

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