Apple MdMYB306-like inhibits anthocyanin synthesis by directly interacting with MdMYB17 and MdbHLH33

文献类型： 外文期刊

第一作者： Wang, Shuo

作者： Wang, Shuo;Zhang, Zhen;Li, Li-Xian;Chen, Xue-Sen;Feng, Shou-Qian;Wang, Hai-Bo;Zhou, Hui

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关键词： apple; anthocyanin; MdMYB306-like; R2R3-MYB repressors; MdMYB17; MdbHLH33

期刊名称：PLANT JOURNAL （ 影响因子：6.486； 五年影响因子：7.666 ）

ISSN： 0960-7412

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收录情况： SCI

摘要： Anthocyanin is one of the most important pigments and nutrients in fruits. Genes encoding R2R3-MYB transcription factors (TFs) are key to anthocyanin regulation. R2R3-MYB activators have been widely studied in the anthocyanin synthesis pathway. However, the mechanism by which R2R3-MYB repressors negatively regulate anthocyanin synthesis remains poorly understood. In the present study, we characterized a subfamily 1 R2R3-MYB anthocyanin repressor gene, MYB306-like, from apple (Malus x domestica) and demonstrated its significance in anthocyanin regulation. The MdMYB306-like protein activates the expression of an anthocyanin repressor gene, MdMYB17, and inhibits the expression of MdDFR (an anthocyanin structural gene) by directly binding to the corresponding promoters. The MdMYB306-like protein interacts with MdMYB17 and MdbHLH33 through its N-terminus. In addition, MdMYB306-like interacts with MdbHLH33 and MdMYB17 to enhance its regulatory activities on MdMYB17 and MdDFR, respectively. Our results revealed that the MdMYB306-like protein, in conjunction with MdMYB17 and MdbHLH33, forms a key regulatory module to fine-tune anthocyanin synthesis in apple.

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