A Lipase Gene of Thermomyces lanuginosus: Sequence Analysis and High-Efficiency Expression in Pichia pastoris

文献类型： 外文期刊

第一作者： Yi, Le

作者： Yi, Le;Cheng, Lifeng;Yang, Qi;Duan, Shengwen;Luo, Wei

作者机构：

关键词： Thermomyces lanuginosus; Pichia pastoris; lipase; heterologous expression; bioinformatics analysis

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：4.9； 五年影响因子：5.7 ）

ISSN： 1661-6596

年卷期： 2024 年 25 卷 21 期

页码：

收录情况： SCI

摘要： Lipase, a type of enzyme that decomposes and synthesizes triglycerides, plays an important role in lipid processing. In this study, a heat-resisting lipase gene (lip4) from Thermomyces lanuginosus was subcloned into the pPICZ alpha A vector and then transformed into Pichia pastoris X33. The recombinant yeast cell concentration reached the maximum (119.5 g/L) at 144 h, and the lipase (Lip4) activity reached the maximum (3900 U/mL) at 168 h in 10 L bioreactor. Through bioinformatics analysis, S168, as the key site of Lip4, participated in the formation of the catalytic triads S168-D223-H280 and G166-H167-S168-L169-G170. Furthermore, S168 and seven conserved amino acids of G104/288, S105, A195, P196, V225 and I287 constitute the active center of Lip4. Specifically, the structure modeling showed two alpha-helices of the lid domain, outside the active pocket domain, controlling the entry of the substrate on Lip4. The potential glycosylation of Asn-33 may be involved in exhibiting the high stable temperature for lipase activity. Therefore, the eukaryotic system was constructed to express Lip4 efficiently, and the amino acid sites related to the catalytic efficiency of Lip4 were clarified, providing a new way for its subsequent property research and industrial application.

分类号：

  • 相关文献
作者其他论文 更多>>
  • A compendium of 8,176 bat RNA viral metagenomes reveals ecological drivers and circulation dynamics

    作者：Yan, Xiaomin;Liu, Yang;Li, Chenxi;Liu, Yuhang;Li, Nan;Sun, Yue;Yi, Le;Tu, Changchun;He, Biao;Hu, Tingsong;Zhang, Fuqiang;Huang, Zhenglanyi;Feng, Jiang;Jiang, Tinglei;Guo, Lei;Zhang, Hailin;Wu, Jianmin;Tu, Changchun

    关键词：

  • Identification of characteristic lipids of Tan lamb and their potential health benefits

    作者：Xu, Le;Li, Shaobo;Chen, Pengyu;Gu, Minghui;Yang, Qi;Chen, Li;Zhang, Dequan

    关键词：Lipid profile; Sheep; Lipid species; Precision nutrition; Functional lipids

  • Natural fatal infection of Tembusu virus in bottlenose dolphins in Thailand

    作者：Piewbang, Chutchai;Zahro, Aisyah Nikmatuz;Poonsin, Panida;Panyathi, Panitnan;Techangamsuwan, Somporn;Piewbang, Chutchai;Zahro, Aisyah Nikmatuz;Poonsin, Panida;Panyathi, Panitnan;Techangamsuwan, Somporn;Yi, Le;He, Biao;Zahro, Aisyah Nikmatuz;Kasantikul, Tanit;Kosoltanapiwat, Nathamon

    关键词：Dolphin; Flavivirus; Interspecies transmission; Tembusu virus; Thailand

  • Exploring the genetic basis of Newcastle disease virus in chickens: a comprehensive review

    作者：Berihulay, Haile;Luo, Wei;Lao, Ainong;Ji, Jian;Cai, Manshan;Shu, Dingming;Luo, Chenglong;Berihulay, Haile

    关键词：chicken; immune response; molecular structure; NDV; pathogenesis; genetic resistance

  • A Fusion XGBoost Approach for Large-Scale Monitoring of Soil Heavy Metal in Farmland Using Hyperspectral Imagery

    作者：Li, Xuqing;Gu, Huitao;Tang, Ruiyin;Chen, Xuying;Song, Yubin;Luo, Wei;Wen, Bin;Li, Xuqing;Ou, Huiping;Li, Xuqing;Zou, Bin;Liu, Xiangnan

    关键词：hyperspectral image; spectral response features; soil heavy metal; farmland; XGB-Boruta; XGBoost

  • Genetic regulation of gene expression across multiple tissues in chickens

    作者：Guan, Dailu;Wang, Ying;Zhou, Huaijun;Bai, Zhonghao;Zhu, Di;Li, Houcheng;Buitenhuis, Albert Johannes;Sahana, Goutam;Lund, Mogens Sando;Fang, Lingzhao;Zhu, Xiaoning;Zhu, Di;Wang, Yuzhe;Hu, Xiaoxiang;Zhong, Conghao;Lan, Fangren;Li, Xiaochang;Sun, Congjiao;Yang, Ning;Zhong, Conghao;Lan, Fangren;Li, Xiaochang;Sun, Congjiao;Yang, Ning;Hou, Yali;Pan, Zhangyuan;Si, Wei;Diao, Shuqi;Gao, Yahui;Teng, Jinyan;Xu, Zhiting;Lin, Qing;Li, Zhenhui;Nie, Qinghua;Zhang, Xiquan;Zhang, Zhe;Yao, Yuelin;Yao, Yuelin;Zhao, Bingru;Gao, Yahui;Liu, George E.;Gao, Yahui;Zou, Dong;Wang, Ruizhen;Xu, Tianyi;Zhang, Zhang;Zou, Dong;Wang, Ruizhen;Xu, Tianyi;Zhang, Zhang;Wang, Ruizhen;Zhang, Zhang;Yin, Hongwei;Shi, Shourong;Shao, Dan;Degalez, Fabien;Lagarrigue, Sandrine;Wang, Mingshan;Peng, Minsheng;Wang, Mingshan;Peng, Minsheng;Rocha, Dominique;Charles, Mathieu;Smith, Jacqueline;Watson, Kellie;Warren, Wesley;Frantz, Laurent;Frantz, Laurent;Larson, Greger;Lamont, Susan J.;Si, Wei;Zhao, Xin;Li, Bingjie;Zhang, Haihan;Luo, Chenglong;Shu, Dingming;Qu, Hao;Luo, Wei;Li, Zhenhui;Nie, Qinghua;Zhang, Xiquan;Li, Zhenhui;Nie, Qinghua;Zhang, Xiquan;Xiang, Ruidong;Xiang, Ruidong;Xiang, Ruidong;Liu, Shuli;Cheng, Hans

    关键词：

  • AtPADRE13 Negatively Regulates Salt Stress Tolerance in Arabidopsis thaliana

    作者：Chang, Ziru;Tian, Xiaona;Niu, Xiaocui;Bai, Meiting;Bai, Wei;Wang, Ruigang;Li, Guojing;Yang, Qi;Tian, Xiaona

    关键词：PADRE; ABA; salt stress; DUF4228; ROS

微信小程序