CsMIKC1 regulates inflorescence development and grain production in Cannabis sativa plants

文献类型： 外文期刊

第一作者： Xu, Gencheng

作者： Xu, Gencheng;Dai, Zhigang;Cheng, Chaohua;Deng, Canhui;Yang, Zemao;Tang, Qing;Su, Jianguang;Zhang, Xiaoyu;Xu, Gencheng;Liu, Yongbei;Li, Chao;Yu, Shuhao;Kong, Dejing;Tang, Kailei;Sun, Jian;Sun, Jian

作者机构：

期刊名称：HORTICULTURE RESEARCH （ 影响因子：7.6； 五年影响因子：8.2 ）

ISSN： 2662-6810

年卷期： 2024 年 11 卷 8 期

页码：

收录情况： SCI

摘要： Female inflorescence is the primary output of medical Cannabis. It contains hundreds of cannabinoids that accumulate in the glandular trichomes. However, little is known about the genetic mechanisms governing Cannabis inflorescence development. In this study, we reported the map-based cloning of a gene determining the number of inflorescences per branch. We named this gene CsMIKC1 since it encodes a transcription factor that belongs to the MIKC-type MADS subfamily. Constitutive overexpression of CsMIKC1 increases inflorescence number per branch, thereby promoting flower production as well as grain yield in transgenic Cannabis plants. We further identified a plant-specific transcription factor, CsBPC2, promoting the expression of CsMIKC1. CsBPC2 mutants and CsMIKC1 mutants were successfully created using the CRISPR-Cas9 system; they exhibited similar inflorescence degeneration and grain reduction. We also validated the interaction of CsMIKC1 with CsVIP3, which suppressed expression of four inflorescence development-related genes in Cannabis. Our findings establish important roles for CsMIKC1 in Cannabis, which could represent a previously unrecognized mechanism of inflorescence development regulated by ethylene.

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