Integrated transcriptomic and proteomic analysis revealed the regulatory role of 5-azacytidine in kenaf salt stress alleviation

文献类型： 外文期刊

第一作者： Luo, Dengjie

作者： Luo, Dengjie;Li, Zengqiang;Mubeen, Samavia;Rehman, Muzammal;Cao, Shan;Wang, Caijin;Yue, Jiao;Pan, Jiao;Chen, Peng;Luo, Dengjie;Li, Ru;Jin, Gang;Chen, Tao;Li, Zengqiang

作者机构：

关键词： Kenaf; Salt stress; Transcriptomics; Proteomics; Correlation analysis

期刊名称：JOURNAL OF PROTEOMICS （ 影响因子：2.8； 五年影响因子：3.0 ）

ISSN： 1874-3919

年卷期： 2024 年 309 卷

页码：

收录情况： SCI

摘要： Salinity stress limits agricultural production. The DNA methyltransferase inhibitor, 5-azacitidine (5-azaC), plays a role in plant abiotic stress regulation, but its molecular basis in mediating salinity tolerance in kenaf remains unclear. To investigate the effects on 5-azaC on alleviating salt stress, kenaf seedlings were pre-treated with 0, 50, 100, 150, and 200 mu M 5-azaC and then exposed to 150 mM NaCl in a nutrient solution. Physiological, transcriptomic, and proteomic analyses were conducted on the root system to understand the regulatory mechanism of 5-azaC (comparing 5-azaC150 and control group 5-azaC0) under salt stress. The results indicated that 5-azaC significantly mitigated salt stress in kenaf by activating the antioxidant system, reducing reactive oxygen species (ROS), and increasing starch, soluble sugars, and adenosine triphosphate (ATP) content. A total of 14,348 differentially expressed genes (DEGs) and 313 differentially abundant proteins (DAPs) were identified. Combined proteomic and transcriptomic analysis revealed 27 DEGs/DAPs, with jointly up-regulated proteins (genes) including HcTHI1, HcBGLU11, and HcCBL1, and jointly down-regulated proteins (genes) including HcGAPDH, HcSS, and HcPP2C52. Overexpression and virus-induced gene silencing (VIGS) of HcPP2C52 demonstrated its role as a negative regulator of salt tolerance. These findings provide insights into the regulatory role of 5-azaC in plant responses to abiotic stresses. Significance: The specific molecular mechanism by which 5-azaC affects gene expression and protein activity of kenaf has been revealed, leading to enhanced salt tolerance.

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