Cold pretreatment enhances microspore embryogenesis in oilseed rape (Brassica napus L.)

文献类型： 外文期刊

第一作者： Gu, HH

作者： Gu, HH;Hagberg, P;Zhou, WJ

作者机构：

关键词： Brassica napus;B. oleracea;B. rapa;Cold pretreatment;Immediate colchicine treatment;Doubled haploid plants;Incubation-temperature;Culture;Induction;Androgenesis;Regeneration;Embryos;Growth;Stress

期刊名称：PLANT GROWTH REGULATION （ 影响因子：3.412； 五年影响因子：3.691 ）

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收录情况： SCI

摘要： Stress is an essential component during embryogenesis induction in microspore culture. Cold pretreatment has been used in cereal microspore culture but very seldom attempted in Brassica microspore culture. The effect of cold pretreatment of flower buds subjected to a liquid medium on microspore embryogenesis was investigated in spring and winter Brassica napus, as well as in B. rapa and B. oleracea. Cold pretreatment significantly enhanced microspore embryogenesis (by 1-7 fold) compared to commonly used microspore culture protocol in B. napus, while it was less effective in B. rapa or even negative in B. oleracea. The appropriate duration of cold pretreatment was found to be 2-4 days, which stimulated the best microspore embryogenesis. Cold pretreatment was also able to promote embryo development including the improvement of embryo quality and acceleration of embryogenesis. When incorporating with medium refreshing, cold pretreatment could initiate the most microspore embryogenesis than any other treatment used. With further improvement cold pretreatment method may have a positive potential in Brassica breeding programmes.

分类号： S

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