A heterophil/lymphocyte-selected population reveals the phosphatase PTPRJ is associated with immune defense in chickens

文献类型： 外文期刊

第一作者： Wang, Jie

作者： Wang, Jie;Zhang, Jin;Wang, Qiao;Zhang, Qi;Thiam, Mamadou;Zhu, Bo;Ying, Fan;Elsharkawy, Mohamed Shafey;Zheng, Maiqing;Wen, Jie;Li, Qinghe;Zhao, Guiping;Wang, Jie

作者机构：

期刊名称：COMMUNICATIONS BIOLOGY （ 影响因子：5.9； 五年影响因子：6.3 ）

ISSN：

年卷期： 2023 年 6 卷 1 期

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收录情况： SCI

摘要： Quantification of leukocyte profiles is among the simplest measures of animal immune function. However, the relationship between H/L ratio and innate immunity and the measure's utility as an index for heterophil function remains to be analyzed. Variants associated with H/L ratio were fine-mapped based on the resequencing of 249 chickens of different generations and an F2 segregating population generated by crossing selection and control lines. H/L ratio in the selection line was associated with a selective sweep of mutations in protein tyrosine phosphatase, receptor type J (PTPRJ), which affects proliferation and differentiation of heterophils through its downstream regulatory genes. The SNP downstream of PTPRJ (rs736799474) have a universal effect on H/L, with CC homozygotes exhibiting improved heterophil function because of downregulated PTPRJ expression. In short, we systematically elucidated the genetic basis of the change in heterophil function resulting from H/L selection by identifying the regulatory gene (PTPRJ) and causative SNP. Genomic and transcriptomic profiling in Jingxing yellow chickens selected for a heterophil-lymphocyte ratio (H/L) provides further insight on the genetic basis of H/L and identifies PTPRJ as a relevant factor.

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