Physiological and transcriptomic comparisons shed light on the cold stress response mechanisms of Dendrobium spp

文献类型： 外文期刊

第一作者： Li, Zhiyuan

作者： Li, Zhiyuan;Zhang, Changqing;Lu, Shunjiao;Yi, Shuangshuang;Mo, Shunjin;Yu, Xiaoyun;Yin, Junmei;Li, Zhiyuan;Zhang, Changqing;Lu, Shunjiao;Yi, Shuangshuang;Mo, Shunjin;Yu, Xiaoyun;Yin, Junmei;Yin, Junmei

作者机构： China Agr Univ, Sanya Inst, Sanya 572025, Hainan, Peoples R China;Chines Acad Trop Agr Sci, Trop Crops Genet Resources Inst, Key Lab Crop Gene Resources & Germplasm Enhancemen, Danzhou 571737, Hainan, Peoples R China;China Agr Univ, Coll Hort, Dept Ornamental Hort, Beijing 100193, Peoples R China;Hainan Engn Ctr Trop Ornamental Plant Germplasm In, Danzhou 571737, Hainan, Peoples R China;Chinese Acad Trop Agr Sci, Sanya Res Inst, Sanya 571101, Peoples R China

关键词： Dendrobium spp; Cold stress; Signaling pathway; WGCNA; Transcriptomic patterns

期刊名称：BMC PLANT BIOLOGY （ 2022影响因子：5.3； 五年影响因子：5.9 ）

ISSN： 1471-2229

年卷期： 2024 年 24 卷 1 期

页码：

收录情况： SCI

摘要： Background Dendrobium spp. comprise a group of tropical orchids with ornamental and medicinal value. Dendrobium spp. are sensitive to low temperature, and the underlying cold response regulatory mechanisms in this group are unclear. To understand how these plants respond to cold stress, we compared the transcriptomic responses of the cold-tolerant cultivar 'Hongxing' (HX) and the cold-sensitive cultivar 'Sonia Hiasakul' (SH) to cold stress. Results Chemometric results showed that the physiological response of SH in the later stages of cold stress is similar to that of HX throughout the cold treatment. Orthogonal partial least squares discriminant analysis (OPLS-DA) revealed that soluble protein content and peroxidase activity are key physiological parameters for assessing the cold tolerance of these two Dendrobium spp. cultivars. Additionally, weighted gene co-expression network analysis (WGCNA) results showed that many cold response genes and metabolic pathways significantly associated with the physiological indices were enriched in the 12 detected modules. The Kyoto Encyclopedia of Genes and Genomes (KEGG) and gene ontology (GO) enrichment analyses of the 105 hub genes showed that Dendrobium spp. adapt to cold stress by regulating signal transduction, phytohormones, transcription factors, protein translation and modification, functional proteins, biosynthesis and metabolism, cell structure, light, and the circadian clock. Hub genes of the cold stress response network included the remorin gene pp34, the abscisic acid signaling pathway-related genes PROTEIN PHOSPATASE 2 C (PP2C), SNF1-RELATED PROTEIN KINASE 2 (SnRK2), ABRE-BINDING FACTOR 1 (ABF1) and SKI-INTERACTING PROTEIN 17 (SKIP17), the Ca(2+ )signaling-related GTP diphosphokinase gene CRSH1, the carbohydrate-related gene STARCH SYNTHASE 2 (SS2), the cell wall biosynthesis gene CINNAMYL ALCOHOL DEHYDROGENASE (CAD7), and the endocytosis-related gene VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 52 A (VPS52A). Conclusions The cold-responsive genes and metabolic pathways of Dendrobium spp. revealed in this study provide important insight to enable the genetic enhancement of cold tolerance in Dendrobium spp., and to facilitate cold tolerance breeding in related plants.

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