Comparative transcriptome analysis reveals genes involved in trichome development and metabolism in tobacco

文献类型： 外文期刊

第一作者： Chen, Mingli

作者： Chen, Mingli;Li, Zhiyuan;Zhang, Zhe;Cui, Luying;Xie, Minmin;Zhao, Zeyu;Gong, Daping;He, Xinxi;Wang, Dong;Zhang, Zhe;Sun, Quan;Wang, Dahai;Dai, Jiameng

作者机构：

关键词： Glandular trichomes; Tobacco; Secondary metabolism; Development; Transcription factors

期刊名称：BMC PLANT BIOLOGY （ 影响因子：4.3； 五年影响因子：5.2 ）

ISSN： 1471-2229

年卷期： 2024 年 24 卷 1 期

页码：

收录情况： SCI

摘要： Background The glandular trichomes of tobacco (Nicotiana tabacum) can efficiently produce secondary metabolites. They act as natural bioreactors, and their natural products function to protect plants against insect-pests and pathogens and are also components of industrial chemicals. To clarify the molecular mechanisms of tobacco glandular trichome development and secondary metabolic regulation, glandular trichomes and glandless trichomes, as well as other different developmental tissues, were used for RNA sequencing and analysis.Results By comparing glandless and glandular trichomes with other tissues, we obtained differentially expressed genes. They were obviously enriched in KEGG pathways, such as cutin, suberine, and wax biosynthesis, flavonoid and isoflavonoid biosynthesis, terpenoid biosynthesis, and plant-pathogen interaction. In particular, the expression levels of genes related to the terpenoid, flavonoid, and wax biosynthesis pathway mainly showed down-regulation in glandless trichomes, implying that they lack the capability to synthesize certain exudate compounds. Among the differentially expressed genes, 234 transcription factors were found, including AP2-ERFs, MYBs, bHLHs, WRKYs, Homeoboxes (HD-ZIP), and C2H2-ZFs. These transcription factor and genes that highly expressed in trichomes or specially expressed in GT or GLT. Following the overexpression of R2R3-MYB transcription factor Nitab4.5_0011760g0030.1 in tobacco, an increase in the number of branched glandular trichomes was observed.Conclusions Our data provide comprehensive gene expression information at the transcriptional level and an understanding of the regulatory pathways involved in glandular trichome development and secondary metabolism.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序