A DNA-launched reverse genetics system for rabbit hemorrhagic disease virus reveals that the VP2 protein is not essential for virus infectivity

文献类型： 外文期刊

第一作者： Liu, Guangqing

作者： Liu, Guangqing;Ni, Zheng;Yun, Tao;Yu, Bin;Chen, Liu;Zhao, Wei;Hua, Jionggang;Chen, Jianping

作者机构：

关键词： FELINE CALICIVIRUS;CAPSID PROTEIN;GENOME ORGANIZATION;CDNA CONSTRUCTS;IN-VITRO;IDENTIFICATION;RNA;EXPRESSION;RECOVERY;TRANSLATION

期刊名称：JOURNAL OF GENERAL VIROLOGY （ 影响因子：3.891； 五年影响因子：3.719 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Rabbit hemorrhagic disease virus (RHDV), a member of the family Caliciviridae comprising positive-stranded RNA viruses, is a highly virulent pathogen of rabbits. Until recently, studies into the molecular mechanisms of RHDV replication and pathogenesis have been hindered by the lack of an in vitro culture system and reverse genetics. This study describes the generation of a DNA-based reverse genetics system for RHDV and the subsequent investigation of the biological role of the RHDV VP2 protein. The full-length RHDV genome was assembled as a single cDNA clone and placed under the control of the eukaryotic human cytomegalovirus promoter. Transfection of cells with the DNA clone resulted in a clear cytopathic effect and the generation of infectious progeny virions. The reconstituted virus was stable and grew to titres similar to that of the parental virus. Although previous reports have suggested that the minor structural protein (VP2) of other caliciviruses is essential for the production of infectious virions, using the DNA-launch-based RHDV reverse genetics system described here it was demonstrated that VP2 is not essential for RHDV infectivity. Transfection of cells with a cDNA clone of RHDV lacking VP2 resulted in the generation of infectious virions. These studies indicate that the presence of VP2 could reduce the replication of RHDV, suggesting that it may play a regulatory role in the life cycle of RHDV.

分类号： R37

  • 相关文献

[1]Protective immune responses in guinea pigs and swine induced by a suicidal DNA vaccine of the capsid gene of swine vesicular disease virus. Sun, Shi-Qi,Liu, Xiang-Tao,Guo, Hui-Chen,Yin, Shuang-Hui,Shang, You-Jun,Feng, Xia,Liu, Zai-Xin,Xie, Cling-Ge.

[2]Molecular cloning, characterization and expression analysis of natural resistance associated macrophage protein (Nramp) cDNA from turbot (Scophthalmus maximus). Chen, Song-Lin,Zhang, Yu-Xi,Xu, Jian-Yong,Meng, Liang,Sha, Zhen-Xia,Ren, Guo-Cheng.

[3]Multiple splicing types of OsRIX4, an RAD21 homolog in rice (Oryza sativa L.). Dong HaiTao,Guo XiaoQin,Pei YanXi,Dai ChengEn,Fang YongQi,Tu QiChao,Zhuang JieYun,Zhao Dong,Zheng KangLe,Li DeBao.

[4]Subcellular localization, expression patterns, SNPs and association analyses of the porcine HUMMLC2B gene. Wang, Huan L.,Wang, Heng,Zhu, Zheng M.,Wang, Chen F.,Zhu, Meng J.,Mo, De L.,Yang, Shu L.,Li, Kui.

[5]Rice endophyte Pantoea agglomerans YS19 promotes host plant growth and affects allocations of host photosynthates. Feng, Y.,Shen, D.,Song, W..

[6]High expression of capsid protein of red-spotted grouper nervous necrosis virus in an avian cell line requires viral RNA2 non-coding regions. Huang, J. N.,Lin, L.,Weng, S. P.,He, J. G.. 2007

[7]High-level expression and immunogenicity of a porcine circovirus type 2 capsid protein through codon optimization in Pichia pastoris. Tu, Yabin,Wang, Yanqun,Wang, Gang,Wu, Jianan,Liu, Yonggang,Wang, Shujie,Jiang, Chenggang,Cai, Xuehui.

[8]High level resistance to Turnip mosaic virus in Chinese cabbage (Brassica campestris ssp pekinensis (Lour) Olsson) transformed with the antisense NIb gene using marker-free Agrobacterium tumefaciens infiltration. Yu Zhandong,Zhao Shuangyi,He Qiwei.

[9]Synthesis of empty capsid-like particles of Asia I foot-and-mouth disease virus in insect cells and their immunogenicity in guinea pigs. Cao, Yimei,Lu, Zengjun,Sun, Jiachuan,Bai, Xingwen,Sun, Pu,Bao, Huifang,Chen, Yingli,Guo, Jianhong,Li, Dong,Liu, Xiangtao,Liu, Zaixin.

[10]Molecular and biochemical characterization of a distinct tyrosinase involved in melanin production from Aeromonas media. Wan, Xia,Chai, Baozhong,Liao, Yi,Ye, Tao,Shen, Ping,Chen, Xiangdong,Wan, Xia,Su, Ying.

[11]Cloning and characterization of a novel Cry1A toxin from Bacillus thuringiensis with high toxicity to the Asian corn borer and other lepidopteran insects. Liang, Gemei,Li, Haitao,He, Kanglai,Song, Fuping,Feng, Xue,Zhang, Jie,Crickmore, Neil,Huang, Dafang.

[12]Cloning and identification of porcine programmed death 1. Zhou, Yan-Jun,Tong, Guang-Zhi,Peng, Jin-Mei,Tian, Zhi-Jun,An, Tong-Qing,Wang, Yao,Li, Deng-Yun,Chen, Jia-Zeng,Yang, Yong-Qian,Tong, Guang-Zhi,Peng, Jin-Mei,Tong, Guang-Zhi,Liu, Heng-Gui.

[13]Engineered Bacillus thuringiensis GO33A with broad insecticidal activity against lepidopteran and coleopteran pests. Wang, Guangjun,Zhang, Jie,Song, Fuping,Wu, Jun,Feng, Shuliang,Huang, Dafang.

[14]Regulation of bolting and identification of the alpha-tubulin gene family in Brassica rapa L. ssp pekinensis. Zhang, Y. W.,Jin, D.,Xu, C.,Zhang, L.,Guo, M. H.,Zhang, Y. W.,Fang, Z. Y.. 2016

[15]Isolation and characterization of glyphosate-regulated genes in soybean seedlings. Yu, Wancong,Zhang, Rui,Li, Runzhi,Guo, Sandui.

[16]Genetic blueprint of the zoonotic pathogen Toxocara canis. Zhu, Xing-Quan,Zhu, Xing-Quan,Korhonen, Pasi K.,Young, Neil D.,Hall, Ross S.,Jex, Aaron R.,Gasser, Robin B.,Cai, Huimin,Li, Qiye,Min, Jiumeng,Yang, Yulan,Wang, Xiuhua,Fang, Xiaodong,Boag, Peter R.,von Samson-Himmelstjerna, Georg,Boag, Peter R.,Tan, Patrick,Tan, Patrick,Hofmann, Andreas,Sternberg, Paul W..

[17]Cloning, expression, identification and bioinformatics analysis of Rv3265c gene from Mycobacterium tuberculosis in Escherichia coli. Wu, Qiang,Zhou, Peng,Wu, Qiang,Zhou, Peng,Wu, Qiang,Qian, Shiyun,Fan, Zhigang,Fu, Qiongyao,Zhan, Zhinong,Pei, Hua,Qin, Xi. 2011

[18]The genome sequence of the multinucleocapsid nucleopolyhedrovirus of the Chinese oak silkworm Antheraea pernyi. Fan, Qi,Li, Shuying,Wang, Linmei,Zhang, Bo,Ye, Bo,Zhao, Zhenjun,Cui, Liwang.

[19]Molecular characterization of a feline calicivirus isolated from tiger and its pathogenesis in cats. Tian, Jin,Liu, Dafei,Liu, Yongxiang,Wu, Hongxia,Jiang, Yanmei,Zu, Shaopo,Liu, Chunguo,Sun, Xue,Liu, Jiasen,Qu, Liandong,Zu, Shaopo,Sun, Xue,Jiang, Yanmei.

[20]Assessment of the IFN-beta response to four feline caliciviruses: Infection in CRFK cells. Tian, Jin,Zhang, Xiaozhan,Wu, Hongxia,Liu, Chunguo,Liu, Jiasen,Hu, Xiaoliang,Qu, Liandong.

作者其他论文 更多>>

微信小程序