Study on the Degradation of Aflatoxin B1 by Myroides odoratimimus 3J2MO

文献类型： 外文期刊

第一作者： Wang, Xue

作者： Wang, Xue;Wang, Dun;Wang, Hao-Ran;Zhang, Ting-Ting;Zhu, Meng-Jie;Dong, Jing;Ling, Dong;Feng, Peng;Tang, Xue-Hui;Wang, Xue;Wang, Dun;Zhang, Qi;Zhu, Meng-Jie;Li, Pei-Wu;Wang, Xue;Gao, Yao-Yao;Wang, Dun;Zhang, Qi;Zhu, Meng-Jie;Li, Pei-Wu;Wang, Xue;Gao, Yao-Yao;Wang, Dun;Zhang, Qi;Zhu, Meng-Jie;Li, Pei-Wu;Zhang, Qi;Zhu, Meng-Jie

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关键词： aflatoxin; helicase; peroxidase; biodegradation

期刊名称：BIOLOGY-BASEL （ 影响因子：3.5； 五年影响因子：4.0 ）

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年卷期： 2025 年 14 卷 6 期

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收录情况： SCI

摘要： To address the issue of aflatoxin contamination, which poses a significant threat to food safety and human health, we have conducted extensive research. We have isolated a strain of Myroides odoratimimus (3J2MO) from the soil that exhibited remarkable efficiency in degrading various aflatoxin types, including AFB1, AFB2, AFG1, AFG2, and AFM1. SDS-PAGE analysis confirmed the purity of the enzymes to be over 95%. Through fluorescence assays, we quantified the enzymatic activity, with an AFB1 degradation rate of 95% achieved at 37 degrees C and a pH of 8.0. Further analysis using HPLC-MS/MS identified the degradation intermediates, revealing the mechanisms of lactone ring cleavage and epoxy group hydrolysis. GO/COG/KEGG annotations provided insights into the functions of these enzymes, with peroxidase linked to reactive oxygen species (ROS) generation and helicase associated with ATP-dependent conformational changes. Helicase, on the other hand, hydrolyzes ATP, driving conformational changes in AFB1 and facilitating its breakdown into non-toxic metabolites. The potential industrial-scale application of this discovery could significantly mitigate aflatoxin-related economic losses while minimizing chemical residues in the food chain.

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