Dietary Zn proteinate with moderate chelation strength alleviates heat stress-induced intestinal barrier function damage by promoting expression of tight junction proteins via the A20/NF-κB p65/MMP-2 pathway in the jejunum of broilers

文献类型： 外文期刊

第一作者： Hu, Yangyang

作者： Hu, Yangyang;Zhang, Weiyun;Cui, Xiaoyan;Li, Tingting;Wang, Shengchen;Hu, Yun;Luo, Xugang;Yang, Ke;Li, Sufen;Lin, Xi;Liu, Hsiao-Ching;Odle, Jack;See, Miles Todd;Liao, Xiudong;Zhang, Liyang

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关键词： A20/NF-kappa B p65/MMP-2 pathway; Broiler; Heat stress; Intestinal barrier function; Tight junction proteins; Zn

期刊名称：JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY （ 影响因子：6.5； 五年影响因子：7.2 ）

ISSN： 1674-9782

年卷期： 2024 年 15 卷 1 期

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收录情况： SCI

摘要： BackgroundThe aim of this study was to determine whether and how Zn proteinate with moderate chelation strength (Zn-Prot M) can alleviate heat stress (HS)-induced intestinal barrier function damage of broilers. A completely randomized design was used for comparatively testing the effects of Zn proteinate on HS and non-HS broilers. Under high temperature (HT), a 1 (Control, HT-CON) + 2 (Zn source) x 2 (added Zn level) factorial arrangement of treatments was used. The 2 added Zn sources were Zn-Prot M and Zn sulfate (ZnS), and the 2 added Zn levels were 30 and 60 mg/kg. Under normal temperature (NT), a CON group (NT-CON) and pair-fed group (NT-PF) were included.ResultsThe results showed that HS significantly reduced mRNA and protein expression levels of claudin-1, occludin, junctional adhesion molecule-A (JAMA), zonula occludens-1 (ZO-1) and zinc finger protein A20 (A20) in the jejunum, and HS also remarkably increased serum fluorescein isothiocyanate dextran (FITC-D), endotoxin and interleukin (IL)-1 beta contents, serum diamine oxidase (DAO) and matrix metalloproteinase (MMP)-2 activities, nuclear factor kappa-B (NF-kappa B) p65 mRNA expression level, and protein expression levels of NF-kappa B p65 and MMP-2 in the jejunum. However, dietary supplementation with Zn, especially organic Zn as Zn-Prot M at 60 mg/kg, significantly decreased serum FITC-D, endotoxin and IL-1 beta contents, serum DAO and MMP-2 activities, NF-kappa B p65 mRNA expression level, and protein expression levels of NF-kappa B p65 and MMP-2 in the jejunum of HS broilers, and notably promoted mRNA and protein expression levels of claudin-1, ZO-1 and A20.ConclusionsOur results suggest that dietary Zn, especially 60 mg Zn/kg as Zn-Prot M, can alleviate HS-induced intestinal barrier function damage by promoting the expression of TJ proteins possibly via induction of A20-mediated suppression of the NF-kappa B p65/MMP-2 pathway in the jejunum of HS broilers.

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