Rapid identification of escolar (Lepidocybium flavobrunneum) and oilfish (Ruvettus pretiosus) species based on recombinase polymerase amplification

文献类型： 外文期刊

第一作者： Xie, Ruibin

作者： Xie, Ruibin;Zhang, Juan;Wen, Shuang;Zhao, Ruiming;Wang, Nan;Chen, Ailiang

作者机构：

关键词： Escolar; Oilfish; Isothermal amplification; Rapid detection; Recombinase polymerase amplification

期刊名称：FOOD CONTROL （ 影响因子：6.0； 五年影响因子：5.8 ）

ISSN： 0956-7135

年卷期： 2023 年 144 卷

页码：

收录情况： SCI

摘要： Escolar or oilfish being sold as cod is a worldwide concerning food counterfeiting issue. This study established a simple and rapid real-time recombinase polymerase amplification (RPA) assay for oilfish (Ruvettus pretiosus) and escolar (Lepidocybium flavobrunneum) detection. The assay uses RPA primers and a probe designed for the intraspecific conserved and interspecific regions in the COI and Cyt b genes, respectively. The RPA assay was completed within 20 min at 37 degrees C, was specific for either oilfish or escolar species, and showed no cross-reactivity with other cod or salmon fishes. The limit of detection for oilfish and escolar was 9.6 pg/mu L and 48 pg/mu L, respectively. Samples that were cooked or digested retained a detection sensitivity of 1%. This real-time RPA assay is a simple, rapid, reliable, and sensitive method for oilfish or escolar identification which may help control oilfish or escolar adulteration and ensure consumer health.

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