The comprehensive detection of miRNA and circRNA in the regulation of intramuscular and subcutaneous adipose tissue of Laiwu pig

文献类型： 外文期刊

第一作者： Feng, Hui

作者： Feng, Hui;Yousuf, Salsabeel;Liu, Tianyi;Zhang, Xiuxiu;Huang, Wanlong;Li, Ai;Xie, Lingli;Miao, Xiangyang

作者机构：

期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.996； 五年影响因子：5.516 ）

ISSN： 2045-2322

年卷期： 2022 年 12 卷 1 期

页码：

收录情况： SCI

摘要： circRNAs, as miRNA sponges, participate in many important biological processes. However, it remains unclear whether circRNAs can regulate lipid metabolism. This study aimed to explore the competing endogenouse RNA (ceRNA) regulatory network that affects the difference between intramuscular fat (IMF) and subcutaneous fat (SCF) deposition, and to screen key circRNAs and their regulatory genes. In this experiment, we identified 265 differentially expressed circRNAs, of which 187 up-regulated circRNA and 78 down-regulated circRNA in IMF. Subsequently, we annotated the function of DEcircRNA's host genes, and found that DEcircRNA's host genes were mainly involved in GO terms (including cellular response to fatty acids, lysophosphatidic acid acyltransferase activity, R-SMAD binding, etc.) and signaling pathways (fatty acid biosynthesis, Citrate cycle, TGF- beta Signal pathway) related to adipogenesis, differentiation and lipid metabolism. By constructing a circRNA-miRNA network, we screened out DEcircRNA that can competitively bind to more miRNAs as key circRNAs (circRNA_06424 and circRNA_08840). Through the functional annotation of indirect target genes and protein network analysis, we found that circRNA_06424 affects the expression of PPARD, MMP9, UBA7 and other indirect target genes by competitively binding to miRNAs such as ssc-miR-339-5p, ssc-miR-744 and ssc-miR-328, and participates in PPAR signaling pathway, Wnt signaling pathway, unsaturated fatty acid and other signaling pathways, resulting in the difference of fat deposition between IMF and SCF. This study provide a theoretical basis for further research investigating the differences of lipid metabolism in different adipose tissues, providing potential therapeutic targets for ectopic fat deposition and lipid metabolism diseases.

分类号：

  • 相关文献
作者其他论文 更多>>
  • MicrobiomeStatPlots: Microbiome statistics plotting gallery for meta-omics and bioinformatics

    作者：Bai, Defeng;Ma, Chuang;Xun, Jiani;Luo, Hao;Yang, Haifei;Lyu, Hujie;Gai, Anran;Yousuf, Salsabeel;Gao, Yunyun;Wang, Yao;Liu, Yong-Xin;Ma, Chuang;Yang, Haifei;Lyu, Hujie;Zhu, Zhihao;Gai, Anran;Peng, Kai;Xu, Shanshan;Gao, Yunyun

    关键词：bioinformatics; interpretation; microbiome; multi-omics; visualization

  • Genome and transcriptome analysis of the lignite-degrading Trichoderma cf. simile WF8 strain highlights potential degradation mechanisms

    作者：Yao, Jinghua;Chen, Yajuan;Zhuo, Deyu;Xu, Baichao;Yan, Congwei;Li, Wanrong;Xiao, Lei;Chen, Yajuan;Zhuo, Deyu;Chen, Siqiao;Xu, Baichao;Yan, Congwei;Li, Wanrong;Feng, Hui;Deng, Sheng;Wei, Lihui;Daly, Paul;Chen, Siqiao;Chen, Siqiao;Cai, Feng M.;Steindorff, Andrei S.;Druzhinina, Irina S.

    关键词：Lignite biodegradation; Trichoderma; Polyaromatic compounds; Comparative genomics

  • Overexpression of the R2R3-MYB transcription factor GmMYB3a enhances isoflavone accumulation in soybean

    作者：Xu, Zibo;Li, Jingwen;Zhang, Yongqiang;Wang, Ying;Zhu, Youcheng;Liu, Tianyi;Liu, Yajing;Wang, Qingyu;Yan, Fan;Song, Xue;He, Yuxuan

    关键词：

  • EasyMetagenome: A user-friendly and flexible pipeline for shotgun metagenomic analysis in microbiome research

    作者：Bai, Defeng;Xun, Jiani;Ma, Chuang;Luo, Hao;Yang, Haifei;Hou, Huiyu;Lv, Hujie;Wan, Xiulin;Wang, Yao;Yousuf, Salsabeel;Zeng, Meiyin;Zhang, Tianyuan;Gao, Yunyun;Liu, Yong-Xin;Chen, Tong;Ma, Chuang;Yang, Haifei;Cao, Chen;Cao, Xiaofeng;Cui, Jianzhou;Deng, Yuan-Ping;Deng, Zhaochao;Yu, Hao;Zhang, Chunfang;Dong, Wenxin;Dong, Wenxue;Du, Juan;Fang, Qunkai;Fang, Wei;Fang, Yue;Luan, Yaning;Fu, Fangtian;Fu, Min;Fu, Yi-Tian;Gao, He;Ge, Jingping;Guo, Yuhao;Gong, Qinglong;Lou, Wenbo;Gu, Lunda;Yang, Li;Guo, Peng;Hai, Tang;Liu, Hao;He, Jieqiang;He, Zi-Yang;Huang, Can;Ji, Shuai;Jiang, ChangHai;Jiang, Gui-Lai;Jiang, Lingjuan;Jin, Ling N.;Li, Changchao;Kan, Yuhe;Kang, Da;Kou, Jin;Lam, Ka-Lung;Li, Chong;Li, Fuyi;Li, Liwei;Li, Miao;Li, Xin;Li, Ye;Li, Zheng-Tao;Zhu, Chengshuai;Liang, Jing;Mo, Jiayuan;Lin, Yongxin;Liu, Changzhen;Liu, Danni;Zhang, Jing;Chen, Shifu;Liu, Fengqin;Liu, Jia;Liu, Tianrui;Liu, Tingting;Wang, Xinlong;Liu, Xinyuan;Luo, Yuanyuan;Liu, Yaqun;Liu, Bangyan;Liu, Minghao;Lv, Hujie;Ma, Tengfei;Mai, Zongjiong;Niu, Dongze;Pan, Zhuo;Qi, Heyuan;Shi, Zhanyao;Song, Chunjiao;Sun, Fuxiang;Sun, Yan;Tian, Sihui;Wang, Guoliang;Wang, Hongyang;Wang, Hongyu;Wang, Huanhuan;Wang, Jing;Wang, Jun;Wang, Kang;Wang, Leli;Yao, Xiaofang;Wang, Shao-kun;Xiao, Zufei;Xing, Huichun;Xu, Yifan;Yang, Song;Yan, Shu-yan;Zhang, Yi-Bo;Yang, Yuanming;Lei, Yu;Yuan, Zhengrong;Zhang, Chunge;Zhang, Huimin;Zhang, Na;Zhang, Yupeng;Zhang, Zheng;Zhou, Mingda;Zhou, Yuanping;Zhu, Zhihao;Zhu, Lin;Zhu, Yue;Zou, Hongqin;Zuo, Anna;Dong, Wenxuan;Wen, Tao;Chen, Shifu;Chen, Shifu;Li, Guoliang

    关键词：metagenome; microbiome; microbiota; pipeline; visualization

  • Oxidizing the odds: FAD-linked oxidase flavoenzymes empower cotton for cadmium-free harvests

    作者：Malik, Waqar Afzal;Yousuf, Salsabeel;Afzal, Maria;Ali, Mushtaque;Sahu, Sunil Kumar;ul Malook, Saif

    关键词：Abiotic stress; Benzyl Isoalloxazine alkaloid; Flavin adenine dinucleotide oxidase; Flavoenzyme; Hyper accumulator; Metallic hazardous; Phytoremediation; Redox homeostasis

  • Relationships Between Oat Phenotypes and UAV Multispectral Imagery Under Different Water Deficit Conditions by Structural Equation Modelling

    作者：Feng, Yayang;Wang, Guoshuai;Wang, Jun;Zheng, Hexiang;Miao, Xiangyang;Feng, Yayang;Sun, Xiulu;Li, Peng;Wang, Guoshuai;Wang, Jun;Zheng, Hexiang;Miao, Xiangyang;Li, Yan;Jia, Yanhui

    关键词：oat phenotype; water deficit; UAV multispectral imagery; vegetation index; structural equation model (SEM)

  • N-(2-Chloro-4-pyridyl)-N'-phenylurea promotes microspore embryogenesis and plant regeneration in pakchoi (Brassica rapa L. ssp. chinensis)

    作者：Li, Xue;Bai, Yuanzhi;Wang, Nan;Feng, Hui;Li, Xue;Bai, Yuanzhi;Wang, Nan;Feng, Hui;Huang, Zhiyin;Fan, Weiqiang

    关键词：Pakchoi; CPPU; Isolated microspore culture; Embryogenesis

微信小程序