Bacteriophage LHE83 targeting OmpA as a receptor exhibited synergism with spectinomycin against Escherichia coli

文献类型： 外文期刊

第一作者： Zhen, Jianyu

作者： Zhen, Jianyu;Liu, Rui;Man, Cheng;Xu, Shijie;Zhang, Wenxiu;Zou, Ling;Liu, Wenhua;Ni, Hong-Bo;Zou, Ming;Zhang, Xiao-Xuan;Zhang, Can;He, Tao;Wang, Ran

作者机构：

关键词： bacteriophage; receptor binding protein; OmpA; spectinomycin; synergistic effect

期刊名称：POULTRY SCIENCE （ 影响因子：3.8； 五年影响因子：4.1 ）

ISSN： 0032-5791

年卷期： 2024 年 103 卷 5 期

页码：

收录情况： SCI

摘要： Understanding the characteristics of bacteriophages is crucial for the optimization of phage therapy. In this study, the biological and genomic characteristics of coliphage LHE83 were determined and its synergistic effects with different types of antibiotics against E. coli E82 were investigated. Phage LHE83 displayed a contractile tail morphology and had a titer of 3.02 pound 109 9 pfu/mL at an optimal MOI of 0.01. Meanwhile, phage LHE83 exhibited good physical and chemical factors tolerance. The 1step growth analysis revealed a latent period of approx. 10 min with a burst size of 87 pfu/infected cell. Phage LHE83 belongs to the genus Dhakavirus. . Its genome consists of 170,464 bp with a 40% GC content, and a total of 268 Open Reading Frames (ORF) ORF ) were predicted with no detected virulent or resistant genes. ORF 213 was predicted to encode the receptor binding protein (RBP) RBP ) and confirmed by the antibody-blocking assay. Furthermore, a phageresistant strain E. coli E82R was generated by co-culturing phage LHE83 with E. coli E82. Genomic analysis revealed that OmpA served as the receptor for phage LHE83, which was further confirmed by phage adsorption assay using E. coli BL21DOmpA, E. coli BL21DOmpA: OmpA and E. coli BL21:OmpA strains. Additionally, a synergistic effect was observed between phage LHE83 and spectinomycin against the drug-resistant strain E. coli E82. These results provide a theoretical basis for understanding the interactions between phages, antibiotics, and host bacteria, which can assist in the clinical application of phages and antibiotics against drug-resistant bacteria.

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