Comparative Transcriptome Analysis of Bt Resistant and Susceptible Strains in Ostrinia furnacalis (Guenee) (Lepidoptera: Crambidae)

文献类型： 外文期刊

第一作者： Lin, Yaling

作者： Lin, Yaling;Shang, Suqin;Lin, Yaling;Gao, Qing;Wang, Yueqin;Wang, Zhenying;He, Kanglai;Zhang, Tiantao;Gao, Qing

作者机构：

关键词： Ostrinia furnacalis; Bacillus thuringiensis; Cry1F; Cry1Ie; transcriptome; resistance

期刊名称：AGRICULTURE-BASEL （ 影响因子：3.408； 五年影响因子：3.459 ）

ISSN：

年卷期： 2022 年 12 卷 2 期

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收录情况： SCI

摘要： The evolution of target pest population resistance to Bt toxins is the most relevant threat to the sustainability of Bt technology, thus it is necessary to clarify insect resistance mechanisms. Firstly, the resistance level of Asian corn borer was determined by bioassay. After 28 generations selection in the lab, the Crylle-resistant strain (ACB-IeR) developed more than 862-fold resistance to Crylle, and the Cry1F-resistant strain (ACB-FR) developed 961-fold resistance to Cry1F. The results show that long-term exposure to Bt toxins can lead to resistance. Then, we compared the differential expression genes (DEGs) of ACB-FR and ACB-IeR with susceptible strain (ACB-BtS), and analyzed GO function and KEGG pathway through transcriptome sequencing. The comparison showed that in Bt-resistant strains, many genes have a significant down-regulated trend. Several Bt-resistance candidate genes were differentially expressed in both resistant strains. Furthermore, the DEGs were verified by RT-qPCR and showed similar trend. These results provide candidate genes for further research on the Bt resistance mechanism.

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