Loop engineering of a thermostable GH10 xylanase to improve low-temperature catalytic performance for better synergistic biomass-degrading abilities

文献类型： 外文期刊

第一作者： You, Shuai

作者： You, Shuai;Zhang, Fang;Bai, Zhi-Yuan;Shittu, Saidi;Herman, Richard-Ansah;Zhang, Wen-Xin;Wang, Jun;You, Shuai;Wang, Jun;Li, Jing

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关键词： Engineered xylanase; Pretreatment; Synergism; Sugarcane bagasse; Energy consumption

期刊名称：BIORESOURCE TECHNOLOGY （ 影响因子：9.642； 五年影响因子：9.237 ）

ISSN： 0960-8524

年卷期： 2021 年 342 卷

页码：

收录情况： SCI

摘要： Lignocellulosic biorefining for producing biofuels poses technical challenges. It is usually conducted over a long time using heat, making it energy intensive. In this study, we lowered the energy consumption of this process through an optimized enzyme and pretreatment strategy. First, the dominant mutant M137E/N269G of Bispora sp. MEY-1 XYL10C_Delta N was obtained by directed evolution with high catalytic efficiency (970 mL/s center dot mg) and specific activity (2090 U/mg) at 37 degrees C, and thermostability was improved (T50 increased by 5 degrees C). After pretreatment with seawater immersion following steam explosion, bagasse was co-treated with cellulase and M137E/N269G under mild conditions (37 degrees C), the resulting highest yield of fermentable sugars reached 219 mu mol/g of bagasse, 46% higher than that of the non-seawater treatment group, with the highest degree of synergy of 2.0. Pretreatment with seawater following steam explosion and synergistic hydrolysis through high activity xylanase and cellulase helped to achieve low energy degradation of lignocellulosic biomass.

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